Mule Bone: A Comedy of Negro Life is a 1930 play by American authors Langston Hughes and Zora Neale Hurston. The process of writing the play led Hughes and Hurston, who had been close friends, to sever their relationship. Mule Bone was not staged until 1991, when it was produced in New York City by the Lincoln Center Theater.

The play begins in Eatonville, Florida, on a Saturday afternoon with Jim and Dave fighting for Daisy's affection. The two men come to blows, and Jim picks up a hock bone from a mule and knocks Dave out. Jim is arrested and held for trial in Joe Clarke's barn.