Adeno-Associated Virus: Methods and Protocols
Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Prools, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and accessible, Adeno-Associated Virus: Methods and Prools provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.
Adeno-Associated Virus: Methods and Protocols
Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Prools, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Adeno-Associated Virus: Methods and Prools provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.
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Adeno-Associated Virus: Methods and Protocols
462Adeno-Associated Virus: Methods and Protocols
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Product Details
ISBN-13: | 9781617793691 |
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Publisher: | Springer-Verlag New York, LLC |
Publication date: | 10/28/2011 |
Series: | Methods in Molecular Biology , #807 |
Edition description: | 2011 |
Pages: | 462 |
Product dimensions: | 7.01(w) x 10.00(h) x 0.04(d) |
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