Adenovirus: Methods and Protocols
Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Prools calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient prools to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
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Comprehensive and cutting-edge, Adenovirus Methods and Prools, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.
Adenovirus: Methods and Protocols
Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Prools calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient prools to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Adenovirus Methods and Prools, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.
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Adenovirus: Methods and Protocols
247Adenovirus: Methods and Protocols
247Hardcover(3rd ed. 2014)
$109.99
109.99
In Stock
Product Details
ISBN-13: | 9781627036788 |
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Publisher: | Springer-Verlag New York, LLC |
Publication date: | 10/17/2013 |
Series: | Methods in Molecular Biology , #1089 |
Edition description: | 3rd ed. 2014 |
Pages: | 247 |
Product dimensions: | 7.01(w) x 10.00(h) x 0.03(d) |
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