Adoptive Immunotherapy: Methods and Protocols / Edition 1

by Burkhard Ludewig, Matthias W. Hoffmann

Overview

An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental prools for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The prools make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

Product Details

ISBN-13:	9781617375460
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/09/2010
Series:	Methods in Molecular Medicine , #109
Edition description:	Softcover reprint of hardcover 1st ed. 2005
Pages:	500
Product dimensions:	5.98(w) x 9.02(h) x 0.05(d)

Table of Contents

Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection.- A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy.- Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres.- RNA Transfection of Dendritic Cells.- Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System.- Generation of Clinical-Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System.- Adenoviral Transduction of Dendritic Cells.- Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy.- Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells.- Dendritic Cells in Clinical Trials for Multiple Myeloma.- Identification of Tumor-Associated Autoantigens With SEREX.- Application of Proteomics and Protein Analysis for Biomarker and Target Finding for Immunotherapy.- Isolation and Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy.- Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers.- Gene Transfer of MHC-Restricted Receptors.- Immunotherapy With CTL Restricted by Nonself MHC.- Designing TCR for Cancer Immunotherapy.- Isolation and Expansion of Tumor-Specific CD4+T-Cells by Means of Cytokine Secretion.- Methods for the Ex Vivo Characterization of Human CD8+T Subsets Based on Gene Expression and Replicative History Analysis.- Regulatory T-Cells in Antitumor Therapy Isolation and Functional Testing of CD4+CD25+Regulatory T-Cells.- Monoclonal Antibody-Based Strategies in Autoimmunity and Transplantation.- Producing Bispecific and Bifunctional Antibodies.- Antibody-Cytokine Fusion Proteins for the Therapy of Cancer.- Synthesis and Biological Evaluation of a Paclitaxel Immunoconjugate.- Cytotoxic Tumor Targeting With scFv Antibody-Modified Liposomes.- Intravenous Immunoglobulin Treatment for Fibrosis, Atherosclerosis, and Malignant Conditions.- Study of T-Cell Costimulatory Blockade In Vivo at a Single-Cell Level.- Stem Cell Transplantation: Graft-Mediated Antileukemia Effects.- Influence of Radiation Prools on Graft-vs-Host Disease Incidence After Bone-Marrow Transplantation in Experimental Models.- Induction of Chimerism and Tolerance Using Freshly Purified or Cultured Hematopoietic Stem Cells in Nonmyeloablated Mice.- Induction of Mixed vs Full Chimerism to Potentiate GVL Effects After Bone-Marrow Transplantation.- Application of Donor Lymphocytes Expressing a Suicide Gene for Early GVL Induction and Later Control of GVH Reactions After Bone-Marrow Transplantation.

Editorial Reviews

Reviewer: Marion C. Cohen, PhD (SUNY Downstate Medical Center)
Description: This book, part of a series entitled Methods in Molecular Medicine, is a compendium of techniques that are currently being used to develop new treatment strategies that take advantage of what we currently know about the human immune system. Within the series, the format of each chapter generally consists of a discussion of the principle(s) involved in the technique, a list of reagents and equipment, step-by-step laboratory instructions, and tips on troubleshooting and avoiding known pitfalls. Each chapter also has a reference list.
Purpose: According to the editors, the purpose is to provide a collection of state-of-the-art techniques to effectively study the adoptive transfer of tailored antigen-specific immune cells for application to human diseases. While there are many methods books available, this one provides carefully described protocols as well as accompanying notes that give hints on troubleshooting and avoiding possible pitfalls. Thus, the book is a useful addition to the library of a researcher who is trying to develop clinical protocols for treatment.
Audience: The editors suggest that a wide variety of basic scientists and clinicians will benefit from this book. Rather than focusing on the target audience, it is perhaps more important that the techniques that are discussed are applicable to many fields including hematology, oncology, molecular medicine, clinical immunology, biotechnology, molecular immunology, immunology, cell biology, biochemical methods, and biomathematics. The authors of each chapter are experts in the techniques they discuss.
Features: The book presents protocols to be used to study dendritic cells, T cells, monoclonal antibodies, bone marrow transplantation for both clinical and preclinical studies. The chapters relating to these individual topics are grouped together and there is a logical flow to the order of the chapters. In addition to the methods chapters, there are several chapters that do not describe actual techniques but describe clinical applications of these cells and reagents. The figures seem to be appropriate for each chapter. All are black and white and, while color is always nice, they convey the information adequately. Each chapter has a list of references. The latest citation is 2004 and there are relatively few of these.
Assessment: This is a useful techniques book to have in the laboratory. The methods seem well described and the section of notes — the little helpful hints that rarely show up in this kind of book — is a very nice touch. If a laboratory is focused on immunotherapy, this book would make a nice addition to its library.

Doody's Review Service

"...a comprehensive and authoritative collection of currently used prools for adoptive immunotherapy in both pre-clinical and clinical settings." - Immunology News

"...will be not only useful for basic researchers but also clinical scientists aiming to translate basic research to a clinical setting." - Immunology and Cell Biology

From the Publisher