Amino Acid Analysis Protocols / Edition 1

by Catherine Cooper

Overview

Amino acid analysis is widely used in biotechnology, biomedical, and food analysis laboratories. Amino Acid Analysis Prools constitutes a major collection of these indispensable analytical techniques, both classic and cutting-edge, of high utility for answering specific biological questions. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Since results depend heavily on the quality of the sample, most contributors have devoted a section to sample preparation, particularly to the collection and storage of bodily fluids. A new method for desalting samples prior to hydrolysis is also provided. Each method is described in step-by-step detail to ensure successful experimental results, and contains helpful notes on pitfalls to avoid, and variations that enable the methods to be used with different systems.

Up-to-date and highly practical, Amino Acid Analysis Prools offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Product Details

ISBN-13:	9780896036567
Publisher:	Springer-Verlag New York, LLC
Publication date:	09/05/2000
Series:	Methods in Molecular Biology , #159
Edition description:	2000
Pages:	265
Product dimensions:	6.10(w) x 9.20(h) x 0.90(d)

Table of Contents

Amino Acid Analysis.- Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory.- Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis.- Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate.- Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent).- The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection.- Anion Exchange Chromatography and Integrated Amperometric Detection of Amino Acids.- Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane.- Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples.- Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization.- Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography.- Amino Acid Measurement in Body Fluids Using PITC Derivatives.- Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis of Phenylthiocarbamyl Derivatives.- Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Capillary Electrophoretic Determination of 4-Hydroxyproline.- Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization.- Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins.

Editorial Reviews

Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: The frequency of amino acid analysis of biological samples has shifted from the research laboratory (where most sequence information is deduced from DNA data, and, if necessary, confirmed by mass spectroscopy) to the industrial biotechnology environment.
Purpose: The purpose is to present current and new methods, specifically biotechnological applications.
Audience: This book will be of interest to analytical chemists working in this arena.
Features: The book begins with a discussion of basic approaches to amino acid analysis and introduces a number of the other chapters which deal with various methods for post-column derivatization with focused applications. This general technique is a necessity in most cases. New methods of separation such as capillary electrophoresis are presented for specific applications. The use of pulsed amperometric detection, a method introduced for saccharide analysis that does not require additional chemistry, is also reviewed.
Assessment: Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis. Some of the material will also be of value to graduate students and fellows working on problems where this type of analysis may be required.

Doody's Review Service

"Sufficient detail and reference material is provided to allow application by a chemist generally familiar with analytical methods. Choices among the various procedures described will likely be made on an empirical basis."-Doody's Health Sciences Book Review Journal

"...the book presents a timely and detailed methodological reference for a broad range of current techniques used in amino acid analysis. The authors are all experts in the field and the editors (especially Williams) have considerable experience in amino acid analysis. this volume will provide an indispensable tool in any laboratory carrying out amino acid analysis."- Today's Life Science

"...This is a major collection of analytical techniques devoted to the analysis of amino acids in biotechnology, food analysis, and biomedical research, as well as the amino acid content of patient samples in clinical laboratories...This book is highly recommended for both engineers and experienced analysts interested in amino acid analysis; both will benefit from the information contained in this collection. It might also be a helpful resource for those looking for a replacement for old, dedicated amino acid analyzers for physiological samples by methods based on newer technologies and alternative techniques." - Clinical Chemistry

"Up-to-date and highly practical, Amino Acid Analysis Prools offers analytical and clinical chemists as well as a broad range of biological and biomedical investigators a richer compendium of laboratory tools for the productive analysis of both common and uncommon amino acids." - Journal of Chromatographic Science

From the Publisher