Animal Cell Technology: From Biopharmaceuticals to Gene Therapy / Edition 1

by Leda Castilho, Angela Moraes, Elisabeth Augusto, Mike Butler

Overview

Animal Cell Technology: from Biopharmaceuticals to Gene Therapy provides a comprehensive insight into biological and engineering concepts related to mammalian and insect cell technology, as well as an overview of the applications of animal cell technology. Part 1 of the book covers the Fundamentals upon which this technology is based and covers the science underpinning the technology. Part 2 covers the Applications from the production of therapeutic proteins to gene therapy. The authors of the chapters are internationally-recognized in the field of animal cell culture research and have extensive experience in the areas covered in their respective chapters.

Product Details

ISBN-13:	9780415423045
Publisher:	Taylor & Francis
Publication date:	12/13/2007
Pages:	564
Product dimensions:	6.88(w) x 9.69(h) x (d)

About the Author

Leda R. Castilho - Cell Culture Engineering Laboratory, Chemical Engineering Program - CPPE, Federal University of Rio do Janeiro, Brazil

Angela Maria Moraes - School of Chemical Engineering, Department of Biotechnological Processes, State University of Campinas, Brazil

Elisabeth F. P. Augusto - Institute of Technological Research of the State of Sao Paulo, Brazil

Michael Butler - Department of Microbiology, University of Manitoba, Canada

Table of Contents

1. Introduction to Animal Cell Technology
2. Animal Cells: Basic Concepts
3. Cloning and Expression of Heterologous Proteins in Animal Cells
4. Cell Metabolism and its Control in Culture
5. Culture Media for Animal Cells
6. Post-Translational Modification of Recombinant Proteins
7. Mechanisms of Cell Proliferation and Cell Death in Animal Cell Culture In Vitro
8. Mathematical Models for Growth and Product Synthesis in Animal Cell Culture
9. Bioreactors for Animal Cells
10. Monitoring and Control of Cell Cultures
11. Animal Cell Separation
12. Product Purification Processes
13. Quality Control of Biotechnological Products
14. Regulatory Aspects
15. Intellectual Property
16. Recombinant Therapeutic Proteins
17. Monoclonal Antibodies
18. Viral Vaccines: Concepts, Principles and Bioprocesses
19. Bioinsecticides
20. Cell Therapies and Stem Cells
21. Gene Therapy

Editorial Reviews

Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: Animal cell technology is currently used to produce a vast array of biological products for basic research and clinical medicine. For example, animal cells are commonly used to produce recombinant proteins, bioinsecticides, viruses, monoclonal antibodies, and pluripotent stem cells. This book summarizes state-of-the art strategies for optimizing the growth of animal cells in culture, covering topics ranging from expression of heterologous proteins to mathematical models for growth and product synthesis to principles of viral vaccines. The primary focus is on biotechnology, bioengineering, cell biology and biochemistry.
Purpose: According to the editors and authors, the aim of this book is to share advances in animal cell technology with a wide audience of technologists and scientists. The authors are prominent, internationally recognized researchers and academic leaders in this field.
Audience: The book is written for basic science and clinical researchers in both academic institutions and the pharmaceutical industry. The broad range of topics and detailed information will appeal to scientists and technicians interested in cell engineering and biotechnology. Undergraduate and graduate students will appreciate this comprehensive discussion of current approaches to producing high-yield biopharmaceuticals. Mathematicians interested in modeling complex biological systems will also find this book inspirational.
Features: Each chapter provides an introduction, review of the literature, discussion of established procedures, and exciting examples of results. After the table of contents, the editors provide a chapter-by-chapter list of abbreviations and symbols. Each chapter includes an extensive list of references and all key concepts and claims are fully documented. The book is carefully edited and a joy to read. I particularly enjoyed the chapter on regulation of cellular metabolism; the details are fascinating. The black/white illustrations are well done and highlight key points. Beautiful full-color glossy pages engage readers and generate enthusiasm for animal cell technology. A case study that models the growth and production of monoclonal antibody by a "hybridoma" cell line is included in an appendix.
Assessment: This book is written for students and researchers interested in animal cell technology. It is not a protocol or methods book. Rather, the authors discuss basic principles and innovative approaches to industrial-scale production of biological products such as antibodies, viruses, and insecticides. A working knowledge of cell biology and biochemistry is assumed. The chapters on mathematical modeling are unique and will help technicians optimize product production. Future applications of animal cell technology will allow scientists to design new therapies for devastating human diseases. This book will become a standard reference manual for biopharmaceutical companies and investigators using animal cells in culture.

Doody's Review Service

"…an excellent introduction to all aspects of animal cell culture."

Biotechnology Advances

From the Publisher