Antibody Engineering / Edition 2

by Carl A.K. Borrebaeck

Overview

As the field of antibody engineering continues its explosive growth, more and more scientists around the world are striving to keep up with the latest developments. Now in its second edition, Antibody Engineering is the perfect one-stop introduction to state-of-the-art technologies emerging in the field today. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector functions, and plant, mammalian, and bacterial expression vectors and hosts. Antibody Engineering, Second Edition—written by leading experts and now thoroughly updated—is a unique resource for current information on the subject. It will be welcomed by researchers in immunology, biotechnology, molecular biology, and biochemistry.

Product Details

ISBN-13:	9780195091502
Publisher:	Oxford University Press
Publication date:	03/09/1995
Series:	Breakthroughs in Molecular Biology
Edition description:	2nd ed
Pages:	416
Product dimensions:	9.22(w) x 6.30(h) x 0.97(d)

About the Author

University of Lund

Table of Contents

1. Introduction: Recombinant Antibodies: Future TrendsAntibody Structure and Function2. Human Monoclonal Antibodies from V-Gene Repertoiries Expressed on Bacteriophage3. Synthetic Antibodies4. Engineering the Antibody Combining Site by Codon-Based Mutagenesis in a Filamentous Phage Display System5. Human Metaphoric Antibodies from a Phagemid Library6. Strategies for Humanizing Antibodies7. Single-Chain Fv Design and Production by Preparative Folding8. Expressing Antibodies in Escherichia Coli9. Vector and Approaches for the Eukaryotic Expression of Antibodies and Antibody Fusion Proteins10. The Cloning of Hybridoma V Regions for their Ectopic Expression in Intracellular and Intercellular Immunisation11. The Expression of 'Single Chain' Antibodies in Transgenic Plants