Antiviral Methods and Protocols / Edition 1

by Derek Kinchington, Raymond F. Schinazi

Overview

This latest addition to the Methods in Molecular Medicine series, Anti- ral Methods and Prools, is opportune because there is an increasing int- est in discovering compounds that are effective against both chronic and acute viral infections. A number of the methods described in the volume are unp- lished and their inclusion indicates the speed at which this field is moving. This volume is not a review but each chapter contains methods validated by the experts who have spent time in developing the prools. The hallmark of this series is the comprehensive way in which the me- ods are described, which includes a list of all the reagents needed for each prool. Of importance is the section on tips and pitfalls that the authors have discovered while developing their prools. The manual itself is designed to be used by researchers in universities and industry who are familiar with a range of biological techniques but who want to set up quickly a novel assay system. We encourage a dialog between readers and authors, which may also result in useful collaborations.

Product Details

ISBN-13:	9781617370731
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/08/2011
Series:	Methods in Molecular Medicine , #24
Edition description:	2000
Pages:	404
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

About the Author

Kinchington, Derek (St Bartholomew's and Royal London School of Medicine and Dentistry); Schinazi, Raymond F. (Emory Univ)

Table of Contents

Changing Methods for Discovering Antiviral Drugs.- Changing Methods for Discovering Antiviral Drugs.- Laboratory Safety Considerations.- Laboratory Safety Considerations.- Routine Quality Control Testing of Cell Cultures.- Routine Quality Control Testing of Cell Cultures.- Hepatitis Virus.- Hep AD38 Assay.- Hepatitis B Virus Cell Culture Assays for Antiviral Activity.- A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase.- Assays for Measuring Hepadnaviral Supercoiled DNA.- Assays for Hepatitis B Virus DNA-and RNA-Dependent DNA Polymerase Activities.- Development of a Hepatitis C Virus RNA Helicase High Throughput Assay.- Herpesvirus.- Determination of Viral Infectivity.- Antiviral Screening Assays for Human Herpesviruses 6 and 7.- Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture.- Monitoring Drug Resistance for Herpesviruses.- Herpesvirus Protease Assays.- Human Immunodeficiency Virus.- Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV.- Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells.- Assessment of Activity of Topical Virucidal Agents.- Assays That Measure Selective Killing of Virus-Infected Cells.- Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs.- Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase.- RT-PCR for Amplification of Specific Fragments of HIV-1 Genome.- Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase.- Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay.- Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses.- Identifying and Characterizing HIV Protease Inhibitors.- RetroviralIntegrase.- Human Papilloma Virus.- Papillomavirus DNA Replication.- Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells.- Influenza Virus.- Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en.- Virological Methods for the Generation and Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en.

Editorial Reviews

"...will be of great benefit to all involved with antiviral development..." -Antiviral Research

"...Together with some useful general discussions on safety and new approaches to antiviral research this volume offers extremely useful information for both the pharmaceutical industry and the academic researcher."-Microbiology Today

"...the editors are to be congratulated for assembling an impressive array of experts in the field as authors for the chapters and the book should remain a valuable reference work for many years, despite the rapidly moving nature of the discipline." - Journal of Antimicrobial Chemotherapy

From the Publisher

Dozens of research scientists from North America, Asia, and Europe, have contributed articles on evaluating antiviral compounds. Coverage includes drugs used against herpes-viruses, hepatitis viruses, human immunodeficiency viruses, and human papilloma viruses; new techniques for evaluating the compounds, including cellular assays and molecular approaches for identifying drugs that are active against viral enzymes; step-by-step instructions to ensure reproducibility; and discussions of quality testing to avoid contamination by microorganisms. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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