Basic Cell Culture / Edition 2

by J. M. Davis

Overview

The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.

Product Details

ISBN-13:	9780199638536
Publisher:	Oxford University Press
Publication date:	03/21/2002
Series:	Practical Approach Series , #254
Edition description:	REV
Pages:	408
Product dimensions:	9.54(w) x 7.46(h) x 0.90(d)

Table of Contents

List of protocolsAbbreviations1. The cell culture laboratory, Caroline B. Wigley2. Sterilzation, Peter L. Roberts3. Culture media, T. Cartwright and G. P. Shah4. Microscopy of living cells, Ian Dobbie and Daniel Zicha5. Basic cell culture and the maintenance of cell lines, James A. McAterr and John M. Davis6. Primary culture and the establishment of cell lines, Caroline MacDonald7. Specific cell types and their requirements, J. D. Sato, D. W. Barnes, I. Hayashi, J. Hayashi, H. Hoshi, T. Kawamoto, W. L. McKeehan, R. Matsuda, K. Matsuzaki, T. Okamoto, G. Serrero, D. J. Sussman, and M. Kan8. Cloning, John Clarke, Alison J. Porter, Robin Thorpe, and John M. Davis9. The quality control of cell lines and the prevention, detection, and cure of contamination, B. J. Bolton, P. Packer, and A. Doyle10. Good Laboratory Practice in the cell culture laboratory, Barbara Clitherow, Stephen J. Froud, and Jan LukerAppendix 1: List of suppliersAppendix 2: Culture collections and other resource centresAppendix 3: Online resources for cell biology, Julian A. T. DowIndex

Editorial Reviews

"If you are planning to set up any functional cell structure facility, small- or large-scale, Basic Cell Structure could be very useful. The book has high practical and scientific value. . .The text is compact and it is easy to find specific information. . .Overall, the book provides a guide for any university of pharmaceutical company laboratory, with or without good laboratory practise (GLP) standards, that aims to obtain consistent cell biological data. I suggest this book cold serve as a handy reference guide and recommend it for managers of cell structure facilities and any person practising cell structure work. . .[T]his book takes on a wider and more conclusive attitude to the cell structure process. Personally, I have placed it in the lab where I am working in , and my colleagues and I have found it to be very handy when working at the bench or planning future studies."—Journal of Cell Science

"The second edition contains a new chapter—on microscopy—updated information throughout the text, and an appendix of Internet sites. Students and professionals in the field of biotechnology will find this a comprehensive, clearly organized guide to laboratory procedures. Each chapter outlines basic principles, detailed discussion of the topic, a list of references, and numerous diagrams and photos. The topics include quality control of cell lines, cloning, specific cell types and their requirements, basic cell culture technique, primary culture, and the establishment and maintenance of cell lines."—SciTech Book News

From the Publisher