Bone Marrow Processing and Purging: a Practical Guide
Bone Marrow Processing and Purging: A Practical Guide provides an up-to-date practical guide to the major ex vivo procedures associated with bone marrow transplantation. Previously, this information was communicated primarily by word of mouth; now experts in the field present detailed descriptions and evaluations of methods for marrow harvesting, evaluation (including tumor infiltration, flow cytometric analysis, and colony assays), comparative methods for automated nucleated cell separation and enumeration, tumor cell purging, T cell depletion, stem cell selection, gene transfer, and cytopreservation. Special sections address quality control and FDA regulations.

The book provides a unique information source intended for clinicians, researchers, technical staff, transplant nurses, and medical students involved in this rapidly expanding area of medicine.
"1136647155"
Bone Marrow Processing and Purging: a Practical Guide
Bone Marrow Processing and Purging: A Practical Guide provides an up-to-date practical guide to the major ex vivo procedures associated with bone marrow transplantation. Previously, this information was communicated primarily by word of mouth; now experts in the field present detailed descriptions and evaluations of methods for marrow harvesting, evaluation (including tumor infiltration, flow cytometric analysis, and colony assays), comparative methods for automated nucleated cell separation and enumeration, tumor cell purging, T cell depletion, stem cell selection, gene transfer, and cytopreservation. Special sections address quality control and FDA regulations.

The book provides a unique information source intended for clinicians, researchers, technical staff, transplant nurses, and medical students involved in this rapidly expanding area of medicine.
281.49 In Stock
Bone Marrow Processing and Purging: a Practical Guide

Bone Marrow Processing and Purging: a Practical Guide

by Adrian P. Gee
Bone Marrow Processing and Purging: a Practical Guide

Bone Marrow Processing and Purging: a Practical Guide

by Adrian P. Gee

eBook

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Overview

Bone Marrow Processing and Purging: A Practical Guide provides an up-to-date practical guide to the major ex vivo procedures associated with bone marrow transplantation. Previously, this information was communicated primarily by word of mouth; now experts in the field present detailed descriptions and evaluations of methods for marrow harvesting, evaluation (including tumor infiltration, flow cytometric analysis, and colony assays), comparative methods for automated nucleated cell separation and enumeration, tumor cell purging, T cell depletion, stem cell selection, gene transfer, and cytopreservation. Special sections address quality control and FDA regulations.

The book provides a unique information source intended for clinicians, researchers, technical staff, transplant nurses, and medical students involved in this rapidly expanding area of medicine.

Product Details

ISBN-13: 9781000141894
Publisher: CRC Press
Publication date: 11/25/2020
Sold by: Barnes & Noble
Format: eBook
Pages: 408
File size: 4 MB

About the Author

Adrian P. Gee

Table of Contents

PART 1: INTRODUCTION AND GENERAL GUIDELINES, Chapter 1: An Introduction to Bone Marrow Transplantation and Processing, Chapter 2: Regulatory Aspects of Bone Marrow Processing and Purging, Chapter 3: Quality Control in Bone Marrow Processing, PART 2: HARVESTING, SHIPPING, AND NUCLEATED CELL SEPARATION, Chapter 4: Bone Marrow Harvesting and Reinfusion, Chapter 5: Transportation of Bone Marrow for In Vitro Processing and Transplantation, Chapter 6: Nucleated Cell Separation Using the Fenwal CS3000™, Chapter 7: Use of the COBE 2991 TM Cell Processor for Bone  Marrow Processing, Chapter 8: Bone Marrow Processing with the Haemonetics V50 Plus™, PART 3: BONE MARROW EVALUATION, Chapter 9: Bone Marrow Cell Counting: Methodological Issues, Chapter 10: Detection of Metastatic Tumor Cells in Bone Marrow, Chapter 11: Flow Cytometric Analysis of Human Bone Marrow, Chapter 12: Quantitative Assays for Human Hemopoietic Progenitor Cells, PART 4: LYMPHOCYTE DEPLETION FOR ALLOGENEIC TRANSPLANTATION, Chapter 13: Lymphocyte Dose Modification of the Bone Marrow Allograft Using Elutriation, Chapter 14: T Cell Depletion of Allogeneic Human Bone Marrow Grafts by Soybean Lectin Agglutination and either Sheep Red Blood Cell Rosetting or Adherence on the CD5/CD8 Cellector™, Chapter 15: Immunotoxin-Mediated Depletion of CDS +T Cells from Bone Marrow for Graft-vs.-Host Disease Prophylaxis, Chapter 16: The Use of Campath Monoclonal Antibodies for Ex Vivo Treatment of Bone Marrow, PART 5: AUTOLOGOUS BONE MARROW PURGING, Chapter 17: The Use of 4-HC in Autologous Purging, Chapter 18: Extracorporeal Purging of Bone Marrow Grafts by Dye-Sensitized Photoirradiation, Chapter 19: Monoclonal Antibodies and Complement for Autologous Marrow Purging, Chapter 20: The Immunomagnetic Manipulation of Bone Marrow, Chapter 21: Combined Purging Approaches in Autologous Transplantation, PART 6: MARROW CRYOPRESERVATION AND FUTURE TRENDS, Chapter 22: Cryopreservation of Human Bone Marrow Grafts, Chapter 23: Simplified Bone Marrow Cryopreservation Using Dimethyl Sulfoxide and Hydroxyethyl Starch as Cryoprotectants, Chapter 24: Selection  of Human Hemopoietic Stem Cells, Chapter 25: Genetic Manipulation of Human Marrow: Gene Transfer Using Retroviruses, INDEX
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