Chemical Approaches to the Synthesis of Peptides and Proteins / Edition 1

by Paul Lloyd-Williams, Fernando Albericio, Ernest Giralt

Overview

Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins. However, more reliable, more efficient synthetic preparation methods have been developed in recent years. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications.
This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides.

Product Details

ISBN-13:	9780849391422
Publisher:	Taylor & Francis
Publication date:	04/23/1997
Series:	New Directions in Organic & Biological Chemistry , #10
Edition description:	New Edition
Pages:	294
Product dimensions:	6.12(w) x 9.19(h) x (d)

About the Author

Paul Lloyd-Williams (Universitat de Barcelona, Barcelona, Spain), Fernando Albericio (Parc Cientific de Barcelona, Spain) and Ernest Giralt (Biomedical Research Institute, Barcelona, Spain)

Table of Contents

IntroductionAmino Acids and Peptide and Protein StructureAmino Acid Activation and CouplingProtection Schemes for Peptide SynthesisThe Synthesis of PeptidesSolid-Phase Peptide SynthesisThe Solid SupportProtection SchemesAttachment of the First Amino Acid to the Solid SupportChain ElongationCleavage of the Peptide from the ResinExamples of Protein Synthesis by Linear SPPSPeptide Synthesis in SolutionStrategic ConsiderationsProtection SchemesChain ElongationFinal Removal of Protecting GroupsSpecial Approaches to the Synthesis of Peptides in SolutionExamples of Peptide Synthesis in SolutionConvergent Approaches to the Synthesis of Large Peptides and ProteinsConvergent Solid-Phase Peptide SynthesisThe Coupling of Protected Peptide Segments in SolutionCoupling of Minimally Protected Peptides in Aqueous SolutionChemical Ligation of Peptide SegmentsFormation of Disulfide BridgesPeptides with Disulfide BridgesProtection of Cysteine in Peptide SynthesisChemical Methods for the Formation of Disulfide BridgesRegioselective Disulfide Bridge FormationPeptide LibrariesMultiple Peptide Synthesis in ParallelPeptide Library TechniquesExamples of Peptide Library SynthesisiEach chapter contains References