Confocal Microscopy: Methods and Protocols / Edition 2

by Stephen W. Paddock

Overview

Confocal Microscopy: Methods and Prools, Second Edition takes the researcher from the bench top through the imaging process, to the page. Prools for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Confocal Microscopy: Methods and Prools, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.

Product Details

ISBN-13:	9781588293510
Publisher:	Springer New York
Publication date:	09/20/2013
Series:	Methods in Molecular Biology , #1075
Edition description:	2nd ed. 2014
Pages:	381
Product dimensions:	7.01(w) x 10.00(h) x (d)

About the Author

Paddock, Stephen W. (Univ of Wisconsin-Madison)

Table of Contents

Reflecting on Confocal Microscopy: A Personal Perspective.- Laser Scanning Confocal Microscopy: History, Applications And Related Optical Sectioning Techniques.- Confocal Microscopy on the Internet.- Using Photoshop with Images Created by a Confocal System.- Clearing up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy.- Low Magnification Confocal Microscopy of Tumor Angiogenesis.- Confocal Imaging of Butterfly Tissue.- Confocal Microscopy of Cardiac Myocytes.- Confocal Imaging of Fluorescently Labeled Proteins in the Drosophila Larval Neuromuscular Junction.- Confocal Imaging and Three-Dimensional Visualization of Thick Autofluorescent Specimens.- Vital Imaging of Multicellular Spheroids.- Live Confocal Analysis of Mutant and Drug Treated Drosophila Embryos.- Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells.- Measurement in the Confocal Microscope.- A Method for Quantifying Blood Flow Distribution Among The Alveoli of the Lung.- Imaging Tools for Analysis of the Ureteric Tree in the Developing Mouse Kidney.- Evaluating Confocal Microscopy System Performance.

Editorial Reviews

A laboratory-bench reference that walks biomedical researchers and students familiar with visible-light microscopy and the basic use of computers through the stages of imaging techniques that virtually eliminates the out-of-focus fluorescence that makes conventional techniques difficult or suspect. Includes information on sampling and staining samples and on manipulating, presenting, and publishing the realized image. Rather than detail technical aspects, which are described elsewhere and change as new techniques and equipment are brought on-line, focuses on a selection of protocols for the most common plant and animal models used in research. Among these are worms, sea urchins, flies, yeast, frogs, and zebrafish. Except four color plates, the illustrations are in black and white. The references include Web sites. Annotation c. by Book News, Inc., Portland, Or.

Booknews

"This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images. . . Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided. . . In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended."-Quarterly Review of Biology

"...almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and prools for confocal microscopy is therefore very timely...The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money."-Cell Biology International

" This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes...there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively."-Microbiology Today

"...The texts are aimed at the biological user...for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations..."-Ultramicroscopy

"...excellent information as an introductory basis for confocal microscopy."-Cellular and Molecular Biology

From the Publisher