Cryopreservation of Plant Germplasm II / Edition 1

by L.E. Towill, Y.P.S. Bajaj

Overview

Ex situ preservation of germplasm for higher plant species has been accom­ plished using either seeds or clones, but storage of these under typical conditions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of sks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur­ vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is commonplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not haveoccurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry­ opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.

Product Details

ISBN-13:	9783540416760
Publisher:	Springer Berlin Heidelberg
Publication date:	05/28/2002
Series:	Biotechnology in Agriculture and Forestry , #50
Edition description:	2002
Pages:	396
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Section I Cryopreservation of Plant Germplasm.- I.1 Cryopreservation of Plant Germplasm: Introduction and Some Observations.- I.2 Implementing Cryopreservation for Long-Term Germplasm Preservation in Vegetatively Propagated Species.- Section II Herbaceous Species.- II.1 Cryopreservation of Allium sativum L. (Garlic).- II.2 Cryopreservation of Apium graveolens L. (Celery) Seeds.- II.3 Cryopreservation of Armoracia rusticana P. Gaert., B. Mey. et Scherb. (Horseradish) Hairy Root Cultures.- II.4 Cryopreservation of Chamomilla recutita L. Rauschert (Chamomie) Callus.- II.5 Cryopreservation of Cichorium intybus L. var foliosum (Chicory).- II.6 Cryopreservation of Colocasia esculenta L. Schott (Taro).- II.7 Cryopreservation of Hordeum (Barley).- II.8 Cryopreservation of Humulus lupulus L. (Hop).- II.9 Cryopreservation of Mentha (Mint).- II.10 Cryopreservation of Panax (Ginseng).- II.11 Cryopreservation of In Vitro Grown Apical Shoot Tips of Wasabia japonica (Wasabi) by Different Procedures.-Section III Woody Species.- III.1 Cryopreservation of Somatic Embryos from Aesculus hippocastanum L. (Horse chestnut).- III.2 Cryopreservation of Azadirachta indica A. Juss. (Neem) Seeds.- III.3 Cryopreservation of Coffee (Coffee).- III.4 Cryopreservation of Eucalyptus spp. Shoot Tips by the Encapsulation-Dehydration Procedure.- III.5 Cryopreservation of Guazuma crinita Mart. (Guazuma).- III.6 Cryopreservation of Olea europaea L. (Olive).- III.7 Cryopreservation of Populus (Poplar) Species.- III.8 Cryopreservation of Prunus.- III.9 Cryopreservation of Quercus (Oak) Species.- III.10 Cryopreservation of Ribes.- III.11 Cryopreservation of Rose (Rose).- Section IV Australian Species.- IV.1 Cryostorage of Somatic Tissue of Endangered Australian Species.- IV.2 Cryopreservation of Australian Species — The Role of Plant Growth Regulators.