Table of Contents
Part I. The Organism, Community Resources, and Genome-wide Studies
The Secret Lives of Dictyostelium Richard H. Kessin
The Genome of Dictyostelium discoideum Adam Kuspa and William F. Loomis
The cDNA Sequencing Project Hideko Urushihara, Takahiro Morio, and Yoshimasa Tanaka
dictyBase and the Dicty Sk Center Petra Fey, Pascale Gaudet, Karen E. Pilcher, Jakob Franke, and Rex L. Chisholm
Analysis of Gene Expression Using cDNA Microarrays Marcel Kaul and Ludwig Eichinger
Proteomic Analysis of Dictyostelium discoideum Udo Roth, Stefan Müller, and Franz-Georg Hanisch
Part II. Basic Methods and Molecular Genetic Techniques
Cultivation, Spore Production, and Mating Hideko Urushihara
Parasexual Genetics Using Axenic Cells Jason King and Robert Insall
Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior Paul R. Fisher and Sarah J. Annesley
Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes Laurence Aubry and Gérard Klein
Generation of Multiple Knockout Mutants Using the Cre-loxP System Alan R. Kimmel and Jan Faix
Restriction Enzyme-Mediated Integration (REMI) Mutagenesis Adam Kuspa
RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium Markus Kuhlmann, Blagovesta Popova, and Wolfgang Nellen
Part III. Imaging and Localization Methods
Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies Annette Müller-Taubenberger
Investigating Gene Expression: In Situ Hybridization and Reporter Genes Ricardo Escalante and Leandro Sastre
Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy Imaging Deborah Wessels, Spencer Kuhl, and David R. Soll
Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Events in Single Living Cells Xuehua Xu, Joseph A. Brzostowski, and Tian Jin
Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development Dirk Dormann and Cornelis J. Weijer
Under-Agarose Chemotaxis of Dictyostelium discoideum David Woznica and David A. Knecht
Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells Monica Hagedorn, Eva M. Neuhaus, and Thierry Soldati
Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum Mark J. Grimson and Richard L. Blanton
Part IV. Dictyostelium as Model Organism
Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro Peter J. M. Van Haastert
Assaying Chemotaxis of Dictyostelium Cells Michelle C. Mendoza and Richard A. Firtel
Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light Scattering Ruth Furukawa and Marcus Fechheimer
Quantitative and Microscopic Methods for Studying the Endocytic Pathway Francisco Rivero and Markus Maniak
Preparation of Intact, Highly Purified Phagosomes from Dictyostelium Daniel Gotthardt, Régis Dieckmann, Vincent Blancheteau, Claudia Kistler, Frank Reichardt, and Thierry Soldati
Assaying Cell-Cell Adhesion Salvatore Bozzaro
Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dictyostelium Cells Mineko Maeda
An Improved Method for Dictyostelium Centrosome Isolation Irene Schulz, Yvonne Reinders, Albert Sickmann, and Ralph Gräf
Epigenetics in Dictyostelium Markus Kaller, Wolfgang Nellen, and Jonathan R. Chubb
Dictyostelium discoideum as a Model to Study Host-Pathogen Interactions Can Ünal and Michael Steinert
Pharmacogenetics: Defining the Genetic Basis of Drug Action and Inositol Trisphosphate Analysis Kathryn E. Adley, Melanie Keim, and Robin S. B.Williams
How to Assess and Study Cell Death in Dictyostelium discoideum Artemis Kosta, Catherine Laporte, David Lam, Emilie Tresse, Marie-Françoise Luciani, and Pierre Golstein
Index