DNA Cloning: A Practical ApproachVolume 1: Core Techniques / Edition 2

by D. M. Glover, B. D. Hames

Overview

This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.

These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Product Details

ISBN-13:	9780199634767
Publisher:	Oxford University Press
Publication date:	06/15/1995
Series:	Practical Approach Series , #148
Edition description:	REV
Pages:	288
Product dimensions:	6.19(w) x 9.25(h) x 0.63(d)

About the Author

University of Dundee

University of Leeds

Table of Contents

1. Techniques for transformation of E.coli2. Lambda replacement vectors in the construction of genomic DNA libraries3. Procedures for cDNA cloning4. Making probes5. Using cloned DNAs to analyse gene organization and expression6. Choices of single-stranded vectors and their application for in vitro mutagenesis7. PCR and its applications8. DNA sequencing

Editorial Reviews

3 Stars from Doody

This book contains the essential information on various basic molecular biology protocols. This second edition is a good reference for a hands-on molecular biology approach. The primary purpose of this and accompanying volumes is as a laboratory reference for molecular biology techniques. This Volume 1 is an excellent reference for laboratories whose primary scope is limited to the genetic aspects of molecular biology. The authors' objectives are thoroughly met. This volume is suitable for persons of varying skill levels using molecular biology techniques. The authors and contributors are credible. The illustrations are a good complement to the text. The references are both current and pertinent as well as thorough. The one unique feature I appreciated most is the font difference between the protocols and the rest of the text. This volume does a very good job in describing the basic or ^^core techniques^^ used in molecular biology laboratories everyday. It offers standard protocols and in some cases alternative strategies to suit the needs of the research project. The hints and troubleshooting portions are helpful, especially for beginners. This would be an excellent addition to a laboratory library.

Chad A. Ellis

Reviewer: Chad A. Ellis(Rush Medical College of Rush University)
Description: This book contains the essential information on various basic molecular biology protocols. This second edition is a good reference for a hands-on molecular biology approach.
Purpose: The primary purpose of this and accompanying volumes is as a laboratory reference for molecular biology techniques. This Volume 1 is an excellent reference for laboratories whose primary scope is limited to the genetic aspects of molecular biology. The authors' objectives are thoroughly met.
Audience: This volume is suitable for persons of varying skill levels using molecular biology techniques. The authors and contributors are credible.
Features: The illustrations are a good complement to the text. The references are both current and pertinent as well as thorough. The one unique feature I appreciated most is the font difference between the protocols and the rest of the text.
Assessment: This volume does a very good job in describing the basic or "core techniques" used in molecular biology laboratories everyday. It offers standard protocols and in some cases alternative strategies to suit the needs of the research project. The hints and troubleshooting portions are helpful, especially for beginners. This would be an excellent addition to a laboratory library.

Doody's Review Service