Drosophila Cells in Culture / Edition 1

by Guy Echalier

Overview

Currently Drosophila is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some thirty years of experience in the handling of in vitro cultured Drosophila cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements.

Product Details

ISBN-13:	9780122294600
Publisher:	Elsevier Science
Publication date:	02/12/1997
Pages:	702
Product dimensions:	6.00(w) x 9.00(h) x (d)

About the Author

Guy Echalier is an esteemed expert in the research of Drosophilia, having over thirty years experience handling in vitro cultured Drosophilia cells.

Table of Contents

Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells
Primary Cell Cultures of Drosophila Cells
Drosophila Continuous Cell Lines
Karyotype and Cell Cycle
Biology and Biochemistry of Cultured Cell Lines: Nucleic Acids
Biology and Biochemistry of Cultured Cell Lines: Proteins
Experimental Models of Gene Regulation: Heat-Shock Response of Drosophila Cells
Experimental Models of Gene Regulation: Cell Responses to Hormones
Gene Transfer Into Cultured Drosophila Cells
Transposons
Drosophila Viruses and Other Infections of Cultured Cells

What People are Saying About This

From the Publisher

New edition of the standard in the field of drosophila cell culture covers experimental procedures and protocols

Editorial Reviews

"Beginners with basic training in handling cultured cells will find the book a useful introduction to the art of culturing Drosophila cells. It is also the definitive reference work on Drosophila cell culture.... May the book inspire many scientists to establish new cell lines, and so help to bridge the gap between vertebrate biochemistry and Drosophila genetics." —NATURE

From the Publisher

Reviewer: Andrew J. Andres, PhD (Northwestern University Feinberg School of Medicine)
Description: This book provides a comprehensive up-to-date source of information concerning the use of cultured Drosophila cells as a research tool.
Purpose: The purpose is to summarize the 30 years of work involved in establishing, maintaining, and using cultured Drosophila cells to span the gap between biochemical and organismal approaches to scientific questions. Its goal is to convince the reader that cultured cells have been and remain a viable and sometimes critical experimental system. This is an important objective, and the author is very convincing.
Audience: Individuals most interested in this book would be practitioners of a research laboratory in a university or industrial setting. The author, a well-known and respected authority in the field, has the unique perspective of being involved in this system from its inception.
Features: The best feature of this book is its comprehensive nature. For example, it has the most complete list of cell lines and media formulation (and how these have evolved over time) that I have ever seen. It also does a very good job of summarizing the past and current literature and listing the important contributions that this system has made to our understanding of gene regulation and developmental genetics. The bibliography itself is invaluable.
Assessment: This book would serve as a crucial reference for anyone using Drosophila as a research organism, or anyone thinking of using Drosophila to assess gene function in an intact organism (i.e., testing vertebrate gene homologues in flies). Every campus library should own a copy, as should every Drosophila research laboratory. Even if a lab is not currently using cell culture, this book would serve those interested in evaluating the feasibility of this system to address a specific question.

Doody's Review Service

3 Stars from Doody

This book provides a comprehensive up-to-date source of information concerning the use of cultured Drosophila cells as a research tool. The purpose is to summarize the 30 years of work involved in establishing, maintaining, and using cultured Drosophila cells to span the gap between biochemical and organismal approaches to scientific questions. Its goal is to convince the reader that cultured cells have been and remain a viable and sometimes critical experimental system. This is an important objective, and the author is very convincing. Individuals most interested in this book would be practitioners of a research laboratory in a university or industrial setting. The author, a well-known and respected authority in the field, has the unique perspective of being involved in this system from its inception. The best feature of this book is its comprehensive nature. For example, it has the most complete list of cell lines and media formulation (and how these have evolved over time) that I have ever seen. It also does a very good job of summarizing the past and current literature and listing the important contributions that this system has made to our understanding of gene regulation and developmental genetics. The bibliography itself is invaluable. This book would serve as a crucial reference for anyone using Drosophila as a research organism, or anyone thinking of using Drosophila to assess gene function in an intact organism (i.e., testing vertebrate gene homologues in flies). Every campus library should own a copy, as should every Drosophila research laboratory. Even if a lab is not currently using cell culture, this book would serve thoseinterested in evaluating the feasibility of this system to address a specific question.

Andrew J. Andres

Currently "Drosophila" is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some 30 years of experience in the handling of "in vitro" cultured "Drosophila" cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements. Annotation c. by Book News, Inc., Portland, Or.

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