Electroporation Protocols for Microorganisms / Edition 1

by Jac A. Nickoloff

Overview

Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Prools for Micro­ organisms describe the underlying theory of electroporation, the com­ mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre­ sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro­ duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ­ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.

Product Details

ISBN-13:	9780896033108
Publisher:	Springer-Verlag New York, LLC
Publication date:	06/20/1995
Series:	Methods in Molecular Biology , #47
Edition description:	1995
Pages:	372
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

About the Author

Nickoloff, Jac A. (Harvard Univ)

The contributors represent the specialties of microbiology, biochemistry, genetics, hygiene and tropical medicine, infectious diseases, immunology, zoology, and biomolecular research. Most are from universities and institutes in the U.S., with some from Canada, Sweden, the U.K., Norway, and Japan. Institutions prominently represented include Univ of Oklahoma Health Sciences Center, Univ of Georgia, Institute for Microbiology in Germany, Univ of Calgary, and London School of Hygiene and Tropical Medicine.

Table of Contents

Electroporation Theory.- Instrumentation.- Direct Plasmid Transfer Between Bacterial Species and Electrocuring.- Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation.- Production of cDNA Libraries by Electroporation.- Electroporation of RNA into Saccharomyces cerevisiae.- Electrofusion of Yeast Protoplasts.- Escherichia coli Electrotransformation.- Electrotransformation in Salmonella.- Electrotransformation of Pseudomonas.- Electroporation of Xanthomonas.- Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids.- Electroporation of Francisella tularensis.- A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation.- Genetic Transformation of Bacteroides spp. Using Electroporation.- Electrotransformation of Agrobacterium.- Electroporation of Helicobacter pylori.- Electrotransformation of Strepocci.- Transformation of Lacoccus by Electroporation.- Transformation of Lactobacillus by Electroporation.- Electrotransformation of Staphylococci.- Electroporation and Efficient Transformation of Enterococcus faecalis Grown in High Concentrations of Glycine.- of Recombinant DNA into Clostridium spp..- Electroporation of Mycobacteria.- Electrotransformation of the Spirochete Borrelia burgdorferi.- Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation.- Ten-Minute Electrotransformation of Saccharomyces cerevisiae.- Electroporation of Schizosaccharomyces pombe.- Gene Transfer by Electroporation of Filamentous Fungi.- Transformation of Candida maltosa by Electroporation.- Electroporation of Physarum polycephalum.- Electroporation of Dictyostelium discoideum.- Gene Transfer by Electroporation in Tetrahymena.- Transfection of the African and American Trypanosomes.- Electroporation in Giardia lamblia.

Editorial Reviews

Reviewer: Mark S. McBride, BS, PhD (University of Wisconsin-Milwaukee)
Description: This book provides comprehensive coverage of the theory and practice of electroporation. The main emphasis is on techniques for introduction of DNA into a wide variety of microorganisms. The chapters are in general well written and up-to-date. There is some overlap between individual chapters, but this is understandable (and in fact essential) given the nature of the book. Each chapter stands on its own to describe conditions for electroporation for a given group of microorganisms.
Purpose: The purpose is to describe the theory of electroporation and provide protocols for the use of electroporation with diverse microorganisms. This is an important text because electroporation is widely used to introduce DNA into microbial cells. This book is undoubtedly the most comprehensive coverage of electroporation of microbial cells available.
Audience: The book is primarily intended for research scientists who require a means to transfer DNA, RNA, or other molecules into microbial cells. This text will be a welcome addition to the library of anyone working in microbial genetics.
Features: The illustrations, index and table of contents are all more than adequate. The only fault (albeit a minor fault) with the book is the number of typographical errors.
Assessment: This is the most comprehensive treatment available on the topic of electroporation of microorganisms The introductory chapters describe the theory and instrumentation of electroporation. The remaining chapters describe protocols for introduction of DNA or other molecules into cells of diverse microorganisms by electroporation. This book will be a useful resource for scientists working on diverse aspects of microbial genetics.

Doody's Review Service

...the most comprehensive treatment available on the topic of electroporation of microorganisms...will be a useful resource for scientists working on diverse aspects of microbial genetics.-Four Stars, Doody's Health Sciences Book Review Journal

From the Publisher

4 Stars! from Doody

This book provides comprehensive coverage of the theory and practice of electroporation. The main emphasis is on techniques for introduction of DNA into a wide variety of microorganisms. The chapters are in general well written and up-to-date. There is some overlap between individual chapters, but this is understandable (and in fact essential) given the nature of the book. Each chapter stands on its own to describe conditions for electroporation for a given group of microorganisms. The purpose is to describe the theory of electroporation and provide protocols for the use of electroporation with diverse microorganisms. This is an important text because electroporation is widely used to introduce DNA into microbial cells. This book is undoubtedly the most comprehensive coverage of electroporation of microbial cells available. The book is primarily intended for research scientists who require a means to transfer DNA, RNA, or other molecules into microbial cells. This text will be a welcome addition to the library of anyone working in microbial genetics. The illustrations, index and table of contents are all more than adequate. The only fault (albeit a minor fault) with the book is the number of typographical errors. This is the most comprehensive treatment available on the topic of electroporation of microorganisms The introductory chapters describe the theory and instrumentation of electroporation. The remaining chapters describe protocols for introduction of DNA or other molecules into cells of diverse microorganisms by electroporation. This book will be a useful resource for scientists working on diverse aspects of microbial genetics.

Mark S. McBride

A laboratory manual for the technique widely used in molecular genetics to introduce DNA into cells for investigations of gene structure and function, and increasingly used for other research purposes such as introducing other types of biological and analytic molecules into cells, to induce cell-to-cell fusion, and to transfer DNA directly between different species. Outlines the protocols for bacterial, fungal, and protist species that are common in research or important to human disease; identifies references to related species; and discusses theory and instrumentation to aid in designing or modifying protocols. Companion volumes will cover plant and animal cells. Claw bound. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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