Table of Contents
Preface iii
Contributors ix
1 Monitoring the Proliferative Capacity of Cultured Animal Cells by Cell Cycle Analysis V. Leelavatcharamas A. Nicholas Emery Mohamed Al-Rubeai 1
2 Use of Flow Cytometry for Monitoring Antibody Productivity and Isolating High-Secreting Hybridoma Cells Katherine L. McKinney Georges Belfort 17
3 Antibody Secretion Assays Using Gel Microdrops and Flow Cytometry James C. Weaver John F. Dunne Forest Gray Kristina G. Lazzari James B. Lin 39
4 High-Resolution Cell Cycle Analysis of Cell Cycle-Regulated Gene Expression Manfred Kubbies Bernhard Goller Guenter Giese 63
5 Stability of Monoclonal Antibody Production in Hybridoma Cell Culture José M. Coco Martin E. Coen Beuvery 85
6 Flow Cytometric Studies of Osmotically Stressed and Sodium Butyrate-Treated Hybridoma Cells Steve Oh Florence K. F. Chua Mohamed Al-Rubeai 101
7 Flow Cytometric Analysis of Cells Obtained from Human Bone Marrow Cultures David Brott Manfred R. Koller Sue A. Rummel Bernhard Palsson 121
8 Measurement of Intracellular pH During the Cultivation of Hybridoma Cells in Batch and Continuous Cultures Jean-Marc Engasser Annie Marc Marc Cherlet Pierre Nabet Patricia Franck 147
9 The Relationship Between Intracellular pH and Cell Cycle in Cultured Animal Cells Using SNARF-1 Indicator Jonathan P. Welsh Mohamed Al-Rubeai 163
10 Assessment of Cell Viability in Mammalian Cell Lines Xavier Ronot Sylvain Paillasson Katherine A. Muirhead 177
11 Analysis of Apoptosis by Flow Cytometry Anne E. Milner Hong Wang Christopher D. Gregory 193
12 Monitoring Baculovirus Infection and Protein Expression in Insect Cells Anthanassia K. Kioukia N. H. Simpson Jatin D. Shah A. Nicholas Emery Mohamed Al-Rubeai 211
13 Use of Protein Distribution to Analyze Budding Yeast Population Structure and Cell Cycle Progression Danilo Porro Lilia Alberghina 225
14 Cell Cycle Analysis of Microorganisms Kirsten Skarstad Rolf Bernander Sture Wold Harold B. Steen Erik Boye 241
15 Bacterial Characterization by Flow Cytometry Gerhard Nebe-von Caron R. Andrew Badley 257
16 Assessment of Viability of Bacteria by Flow Cytometry Clive Edwards 291
17 Advances in the Flow Cytometric Characterization of Plant Cells and Tissues David W. Galbraith Georgina M. Lambert 311
Index 327
