Flow Cytometry: First Principles / Edition 2

by Alice Longobardi Givan

Overview

Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis.

Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:

• Assumes no prior knowledge of flow cytometry
• Progresses with an informal, engaging lecture style from simpleto more complex concepts
• Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis
• Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields

Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Product Details

ISBN-13:	9780471382249
Publisher:	Wiley
Publication date:	07/23/2001
Edition description:	REV
Pages:	304
Product dimensions:	6.30(w) x 9.27(h) x 0.66(d)

About the Author

Alice Givan was born in Brooklyn, N.Y.. She received her A.B. at Bryn Mawr College and her Ph.D. at Harvard University. After completing her formal education, she moved to the north of England where, at Newcastle University Medical School, she learned flow cytometry with an instrument that was being used for the development of methods to predict and monitor rejection reactions in transplant patients. After 20 years in England, Alice Givan moved back to the United States and is now Director of the Herbert C. Englert Cell Analysis Laboratory (the flow cytometry and fluorescence imaging resource of the Norris Cotton Cancer Center) at Dartmouth Medical School. She also organizes and teaches courses and workshops for new flow cytometrists.

Table of Contents

Preface.

Acknowledgments (First Edition).

Acknowledgments (Second Edition).

The Past as Prologue.

Setting the Scene.

Instrumentation: Into the Black Box.

Information: Harnessing the Data.

Seeing the Light: Lasers, Fluorochromes, and Filters.

Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating.

Cells from Within: Intracellular Proteins.

Cells from Within: DNA in Life and Death.

The Sorting of Cells.

Disease and Diagnosis: The Clinical Laboratory.

Out of the Mainstream: Research Frontiers.

Flowing On: The Future.

General References.

Glossary.

Figure Credits.

Index.

What People are Saying About This

From the Publisher

"I consider it to be the best presentation of principles of flow cytometry. The text is clearly written, the figures are well chosen." —Zbigniew Darzynkiewicz, Brander Cancer Research Institute, New York Medical College

Editorial Reviews

"...reflects innovations in the field since the first edition was published..." (SciTech Book News, Vol. 26, No. 2, June 2002)

From the Publisher

Based on a short course Givan has presented for some years at Newcastle University, England, explaining to students and nonspecialists the vocabulary, assumptions, strengths, and weaknesses of flow cytometry and its use in the biological and medical sciences. Could serve as an introduction for those who might actually practice the technique, but mainly for those who will encounter it in their work. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

This second edition of a text for students, scientists, clinicians, and technologists reflects innovations in the field since the first edition was published in 1992, outlining fundamental elements of instrumentation, sample preparation, and data analysis. It explains basic principles of flow cytometry, surveys its primary scientific and clinical applications, and highlights new techniques. This edition offers new discussions on fluorochrome and laser options for multicolor analysis, a new section on apoptosis in the chapter on DNA, and new chapters on intracellular protein staining and cell sorting. No prior knowledge of flow cytometry is assumed. Givan is affiliated with the Norris Cotton Cancer Center and the Department of Physiology at Dartmouth Medical School. Annotation c. Book News, Inc., Portland, OR (booknews.com)