Fluorescence Spectroscopy in Biology: Advanced Methods and their Applications to Membranes, Proteins, DNA, and Cells / Edition 1

by Martin Hof, Rudolf Hutterer, V. Fidler

Overview

Take any combination of the following features: supramolecular structures with a specific fluorescent probe localized as you would like; nanoscale spatial reso- tion; tailor-made molecular and/or solid-state fluorescing nanostructures; us- friendly and/or high- throughput fluorescence techniques; the ability to do wh- ever you wish with just one single (supra)molecule; utilization of non-linear optical processes; and,last but not least,physical understanding of the processes resu- ing in a (biological) functionality at the single molecule level. What you will then have is some recent progress in physics,chemistry,and the life sciences leading to the development of a new tool for research and application. This was amply demonstrated at the 8th Conference on Methods and Applications of Fluorescence: Probes,Imaging,and Spectroscopy held in Prague,the Czech Republic on August 24th–28th, 2003. This formed a crossroad of ideas from a variety of natural science and technical research fields and biomedical applications in particular. This volume – the third book in the Springer-Verlag Series on Fluorescence – reviews some of the most characteristic topics of the multidisciplinary area of fluorescence applications in life sciences either presendted directly at th 8th MAF Conference or considered to be a cruical development in the field. In the initial contribution in Part 1 – Basics and Advanced Approaches,the - itors explain the basics of fluorescence and illustrate the relationship between some modern fluorescence techniques and classical approaches. The second contrigution by B.

Product Details

ISBN-13:	9783540223382
Publisher:	Springer Berlin Heidelberg
Publication date:	01/12/2005
Series:	Springer Series on Fluorescence , #3
Edition description:	2005
Pages:	305
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Fluorescence Spectroscopy: Basics and Advanced Approaches.- Basics of Fluorescence Spectroscopy in Biosciences.- Pulse and Phase Fluorometries: An Objective Comparison.- Non-Exponential Fluorescence of Electronically Coupled Donors Contains Distance Information.- Fluorescence Nanotomography: Recent Progress, Constraints and Opportunities.- Solvent Relaxation as a Tool for Probing Micro-Polarity and -Fluidity.- Total Internal Reflection Fluorescence Microscopy: Applications in Biophysics.- Single Molecule Spectroscopy: Basics and Applications.- Application of Fluorescence Spectroscopy to Biological Membranes.- Raft Microdomains in Model Membranes as Revealed by Fluorescence Quenching.- The Lateral Structure of Lipid Membranes as Seen by Fluorescence Microscopy.- Application of Fluorescence Spectroscopy to Protein Studies.- Protein Dynamics and Protein Folding Dynamics Revealed by Time-Resolved Fluorescence.- Time-Resolved Fluorescence and Two-Photon FCS Investigation of the Interaction of HIV-1 Nucleocapsid Protein with Hairpin Loop Oligonucleotides.- Application of Fluorescence Spectroscopy to DNA and Drug Delivery.- Fluorescence Techniques in Non-Viral Gene Therapy.- Fluorescence Applications in Targeted Drug Delivery.- Fluorescence Spectroscopy in cells: FCS and Quantum Dots.- Fluorescence Correlation Spectroscopy in Cell Biology.- Fluorescent Quantum Dots: Properties and Applications.- Heat Stress of Cancer Cells: Fluorescence Imaging of Structural Changes with Quantum Dots 605 and Alexa 488.