Table of Contents
Foreword (Robert J. Lefkowitz).
Series Preface (David R. Sibley).
Preface (Susan R. George & Brian F. O'Dowd).
Contributors.
1. Purification of the G Protein-Coupled Receptor Rhodopsin for Structural Studies (David Salom, Ning Li, Li Zhu, Izabela Sokal and Krzysztof Palczewski).
2. Cooperativity in Ligand Binding to Deduce Receptor/Receptor Interaction (Meritxell Vivo and Philip G. Strange).
3. Analysis of Receptor-Receptor Interaction Using Time-Resolved Fluorescence Resonance Energy Transfer (Douglas Ramsay and Graeme Milligan).
4. Real-Time Imaging of GPCR-Medicated Arresting Translocation as a Strategy to Evaluate Receptor-Protein Interactions (Robert H. Oakley, Larry S. Barak and Marc G. Caron).
5. Localization of G Protein Coupled Receptors and Interacting Proteins in Membrane Microdomains (K. Saidas Nair, Vijaya Narayanan and Vladlen Z. Slepak).
6. Identification of Peptide Ligand Binding Domains Within G Protein-Coupled Receptors Using Photoaffinity Labeling (Maoqing Dong and Laurence J. Miller).
7. Analysis in Real-Time of the Assembly and Disassembly of Soluble G Protein-Coupled Receptor-G Protein Complexes (Seam M. Biggs, Peter I. Simons, Anna Waller, Terry Founts, Tione Buranda, Eric R. Prossnitz and Larry A. Sklar).
8. Approaches to Studying Membrane Protein Oligomerization: Use of Cysteine Cross-Linking Strategies to Analyze Receptor-Receptor Interactions (Jonathan A. Javitch, Wen Guo and Marisa E. Ring).
9. Co-Immunoprecipitation as a Strategy to Evaluate Receptor-Receptor or Receptor-Protein Interactions (Randy A. Hall).
10. Use of MALDI-TOF and Mass Spectrometry in the Evaluation of Receptor-Protein Interactions (John R. Raymond, Henry Ayiku, Justin Turner, Andrew Gelasco and John M. Arthur).
11. Use of the Yeast Two-Hybrid System to Detect Receptor-Protein Interactions (Ridwan Lin, Nadine Kabbani, Alicia Binda, Victor Canfield and Robert Levenson).
12. GST Fusion Protein and Pull-down Strategies to Evaluate Receptor-Receptor or Receptor-Protein Interactions (Frank J.S. Lee and Fang Liu).
13. G-Protein-Coupled Fusion to Monitor Integral Membrane Protein-Protein Interactions (Henrik Dohlman and Kathleen Ehrhard).
14. Enzyme-Linked Immunosorbent Assays as Tools in Studying Interactions of G-Protein-Coupled Receptors (Torsten Schöneberg, Katrin Sangkuhl, Holger Römpler and Angela Schulz).
15. Electrophysiological Characterization of GPCR and Ligand-Gated Channel Crosstalk (Tak Pan Wong, Xian-Ming Yu and Yu Tian Wang).
16. BRET as a Strategy to Evaluate Receptor-Receptor or Receptor-Protein Interactions (Karen A. Eidne and Kevin D.G. Pfleger).
Index.
