Gene Expression Systems: Using Nature for the Art of Expression / Edition 1

by Joseph M. Fernandez, James P. Hoeffler

Overview

Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression.

Product Details

ISBN-13:	9780122538407
Publisher:	Elsevier Science
Publication date:	11/16/1998
Pages:	480
Product dimensions:	6.00(w) x 9.00(h) x (d)

About the Author

Joseph Fernandez is the Vice President of Business Development at Invitrogen Corporation in Carlsbad, California, where he identifies and licenses new technologies including gene expression systems.

Jim Hoeffler is the Director of Research and Development at Invitrogen Corporation, concentrating on the development of new products, with a focus on gene expression systems.

Table of Contents

Contributors.
Introduction: The Art of Expression.
Purpose of This Book.
Selecting a Suitable Expression System:
Considerations.
Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler.
Prokaryotic Expression Systems:
Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin.
Expression Vectors Employing the trc Promoter, J. Brosius.
Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller.
araB Expression System in Escherichia coli, M. Better.
Eukaryotic Expression Systems:
Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow.
Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg.
Recombinant Protein Expression in Pichia methanolica, C.K. Raymond.
Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski.
Inducible Mammalian Expression Systems, M.Russell.
Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett.
Expression in Insect Systems:
Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman.
Baculovirus Expression Vector System, M. Galleno and A.J. Sick.
Transgenic Expression:
Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson.
Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling.
Recombinant Protein Expression in Plants, A.E. Voloudakis, Y. Yin, and R.N. Beachy.
Index.

Editorial Reviews

Reviewer: Michael Cummings, PhD (University of Illinois at Chicago)
Description: Many researchers in the field of recombinant DNA research are adept at the manipulation of vectors and restriction enzymes, and can produce constructs that combine genetic elements from bacteria, yeast, and mammals in a dazzling array of combinations. Fewer researchers are experienced in the selection and use of a protein expression system. This book contains a comprehensive review of in vitro and in vivo gene expression systems.
Purpose: The reader is given the information needed to select and use the most appropriate gene expression system.
Audience: Of particular value in each chapter are sections in which the advantages and disadvantages of each system are considered, as well as future directions. As a result, this book will be useful to a wide range of readers including academic researchers, industrial scientists seeking to use a protein expression system, and clinicians who wish to learn about gene therapy systems. The chapters are written by authorities in the field, many of whom are in the forefront of gene expression research.
Features: The book is divided into four sections. The first contains four chapters dealing with viral and prokaryotic expression systems, including T7 polymerase, Bacillus and E. coli. In the second section eukaryotic systems are covered, including Pichia and mammalian cells. Section III contains two chapters on the use of insect systems and baculovirus vectors. The last section deals with expression in transgenic mice and the milk of transgenic animals. The advantages and disadvantages of each system are evaluated, and potential problems are addressed, with suggestions for solutions. An opening section of the book contains a table that will allow a researcher to select the systems or system most appropriate for his or her requirements. The chapters cover transient expression systems, inducible systems, stable expression, and large-scale production of a gene product. The authors recognize that expression of cloned genes is a combination of art and science, and the actual and potential pitfalls are acknowledged and discussed.
Assessment: This is the most comprehensive and clearly written book available on gene expression systems, and belongs on the shelf of everyone interested in protein expression.

Doody's Review Service

4 Stars! from Doody

Many researchers in the field of recombinant DNA research are adept at the manipulation of vectors and restriction enzymes, and can produce constructs that combine genetic elements from bacteria, yeast, and mammals in a dazzling array of combinations. Fewer researchers are experienced in the selection and use of a protein expression system. This book contains a comprehensive review of in vitro and in vivo gene expression systems. The reader is given the information needed to select and use the most appropriate gene expression system. Of particular value in each chapter are sections in which the advantages and disadvantages of each system are considered, as well as future directions. As a result, this book will be useful to a wide range of readers including academic researchers, industrial scientists seeking to use a protein expression system, and clinicians who wish to learn about gene therapy systems. The chapters are written by authorities in the field, many of whom are in the forefront of gene expression research. The book is divided into four sections. The first contains four chapters dealing with viral and prokaryotic expression systems, including T7 polymerase, Bacillus and E. coli. In the second section eukaryotic systems are covered, including Pichia and mammalian cells. Section III contains two chapters on the use of insect systems and baculovirus vectors. The last section deals with expression in transgenic mice and the milk of transgenic animals. The advantages and disadvantages of each system are evaluated, and potential problems are addressed, with suggestions for solutions. An opening section of the book contains a table that will allow aresearcher to select the systems or system most appropriate for his or her requirements. The chapters cover transient expression systems, inducible systems, stable expression, and large-scale production of a gene product. The authors recognize that expression of cloned genes is a combination of art and science, and the actual and potential pitfalls are acknowledged and discussed. This is the most comprehensive and clearly written book available on gene expression systems, and belongs on the shelf of everyone interested in protein expression.

Michael Cummings

Guides readers through state-of-the-art protein expression systems and assists in the selection of those that will best suit individual goals and resources. Fifteen articles review the most popular prokaryotic and eukaryotic expression systems, including bacteria, yeast, mammalian cell cultures, and transgenic animals and plants. Topics include expression vectors employing the promoter, adenoviral vectors for protein expression, and baculovirus expression vector systems. Annotation c. by Book News, Inc., Portland, Or.

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