Gene Transfer in the Cardiovascular System: Experimental Approaches and Therapeutic Implications / Edition 1

by Keith L. March

Overview

The goal of gene transfer is protein expression. a process brought about by the insertion of a gene coding for a foreign protein into target cells resulting in the synthesis of the foreign protein For gene therapy, a tmnsferred therapeutic gene must be expressed at a level beneficial for the patient. This chapter provides an introductory overview of the rapidly evolving field of non-viral approaches for gene delivery to rnarnrnalian cells. Although currently there are fewer ongoing clinical trials using non-viral approaches than those using viral based systems, the number of non-viral trials is increasing. The long range goal of some research groups is the development of a genetically engineered artificial virus targeted to specific cells in the human body. An arurual conference, organized by Cambridge Healthtech Institute entitled "Artificial Self-Assembling Systems for Gene Transfer", brings together researchers interested in this field [1]. Assembly of an artificial virus is very complex; other research groups aim to develop simpler delivery systems consisting of a plasmid combined with delivery agents. Viral-based systems are very successful for gene delivery, but despite their successes, viral-based systems have some geneml limitations and system-specific limitations. When employing a viml-based system, the following limitations should be considered: • size limitation of the inserted gene due to packaging constraints (e. g. adenovirus, retrovirus) . • potential tumorigenesis (e. g. retrovirus) • potential for insertional mutagenesis (greater than plasmid based systems) • potential imrnunogenicity (e. g.

Product Details

ISBN-13:	9781461378815
Publisher:	Springer US
Publication date:	10/12/2012
Series:	Developments in Cardiovascular Medicine , #189
Edition description:	Softcover reprint of the original 1st ed. 1997
Pages:	516
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

About the Author

March, Keith L., MD, PhD (Indiana Univ)

The contributors represent the specialties of cardiology, biochemistry, molecular/cell biology, molecular genetics, and vascular medicine. Most are from universities and hospitals in the U.S. and France. Institutions prominently represented include Indiana Univ, Georgetown Univ, and Stanford.

Table of Contents

Vectors and Gene Transfer Systems: Molecular Aspects of Delivery.- 1. Development of Viral Vectors for Human Gene Therapy: Retrovirus and Adenovirus (Part I).- 2. Adenoviruses (Part II): Improvement of Adenoviral Vectors for Human Gene Therapy: E1 and E4 Deleted Recombinant Adenoviruses.- 3. Adeno-associated Virus and Other New DNA Virus Vectors.- 4. Plasmid and Other Non-Viral Vectors.- 5. The HVJ/Liposome Molecular Delivery System for In Vivo Genetic Engineering.- 6. Endogenous Expression Modification: Antisense Approaches.- Methods for Localizing Gene Transfer: Mechanical Aspects of Delivery.- 7. Catheter-Based Local Drug and Gene Delivery.- 8. Fluid Dynamics of Catheter Delivery: Effects on Delivery Efficiency and Localization.- 9. Targeted and Sustained-Release Delivery Concepts in Gene Therapy.- Gene Delivery for Local Vascular Expression.- 10. Viral Vector-Based Vascular Gene Delivery: Basic Studies and Therapeutic Applications.- 11. Cell-Based Vascular Gene Delivery: Endothelial Cells as Carriers.- 12. Cell-Based Gene Delivery: Smooth Muscle Cells as Carriers.- 13. Vascular Cell Proliferation Dynamics: Implications for Gene Transfer and Restenosis.- 14. Angiogenesis and Collateral formation.- Gene Delivery for Local Cardiac Expression.- 15. Cell-Based Myocardial Protein Delivery.- 16. Skeletal Myoblast Therapy in Cardiovascular Disease.- 17. Adenovirus and the Myocardium.- Gene Delivery for Systemic Expression.- 18. Gene Delivery for Systemic Expression: Plasmid, Retroviral, and Adenoviral Approaches.- 19. Adenoviral Gene Delivery Approaches for Systemic Expression.- 20. Experimental Approaches Using Kallikrein Gene Therapy for Hypertension.- Biophysical Considerations in Vector Delivery.- 21.Pharmacokinetics of Local Vector Delivery To Vascular Tissues: Implications for Efficiency and Localization.- Color Plates.

Editorial Reviews

3 Stars from Doody

This is multiauthored monograph contains contributions by experts in the field of cardiovascular gene therapy. The editor, Dr. Keith March, a well-respected cardiovascular scientist in this rapidly developing field, has done an outstanding job of integrating into a unique book contributions from a very diverse group of writers. The overall goal is to incorporate recent information from the entire spectrum of the discipline into a single source. According to the editor, the book is intended to be a helpful resource to cardiovascular scientists and molecular biologists involved in or interested in the field of gene transfer in the cardiovascular system. The book is divided into six parts, containing a total of 21 chapters, and features contributions by academic and industry leaders. To expedite publishing, the book was prepared as camera-ready copy, but it is remarkably easy to read. Despite this fact, references appear to be somewhat dated (only up to 1994 in some chapters). Most chapters are appropriately illustrated with useful black-and-white figures and schematics. Several color plates precede a well-organized index, and there are numerous cross-references within individual chapters to improve readability of this multiauthored work. The overall appearance of the book is very good. It provides a thoughtful overview of a complex subject that offers great promise. The book is an excellent addition to any cardiovascular scientist's library, and it should be a useful resource for those in this rapidly growing field.

Allen M. Samarel

Reviewer: Allen Mark Samarel, MD (Loyola University Stritch School of Medicine)
Description: This is multiauthored monograph contains contributions by experts in the field of cardiovascular gene therapy. The editor, Dr. Keith March, a well-respected cardiovascular scientist in this rapidly developing field, has done an outstanding job of integrating into a unique book contributions from a very diverse group of writers.
Purpose: The overall goal is to incorporate recent information from the entire spectrum of the discipline into a single source.
Audience: According to the editor, the book is intended to be a helpful resource to cardiovascular scientists and molecular biologists involved in or interested in the field of gene transfer in the cardiovascular system.
Features: The book is divided into six parts, containing a total of 21 chapters, and features contributions by academic and industry leaders. To expedite publishing, the book was prepared as camera-ready copy, but it is remarkably easy to read. Despite this fact, references appear to be somewhat dated (only up to 1994 in some chapters). Most chapters are appropriately illustrated with useful black-and-white figures and schematics. Several color plates precede a well-organized index, and there are numerous cross-references within individual chapters to improve readability of this multiauthored work. The overall appearance of the book is very good. It provides a thoughtful overview of a complex subject that offers great promise.
Assessment: The book is an excellent addition to any cardiovascular scientist's library, and it should be a useful resource for those in this rapidly growing field.

Doody's Review Service