General Techniques of Cell Culture

by Maureen A. Harrison, Ian F. Rae

Overview

Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book.

Product Details

ISBN-13:	9780521573641
Publisher:	Cambridge University Press
Publication date:	10/13/1997
Series:	Handbooks in Practical Animal Cell Biology
Pages:	176
Product dimensions:	6.18(w) x 9.29(h) x 0.63(d)

About the Author

Harrison, Maureen A. (Imperial Cancer Research Fund, London); Rae, Ian F. (Imperial Cancer Research Fund, London)

Table of Contents

Preface; 1. Introduction; 2. Equipment; 3. Media; 4. Sera; 5. Cell culture; 6. Preparation of primary cells; 7. Cryopreservation; 8. Quality control; 9. Specialized techniques; 10. Health and safety; Acknowledgements; Glossary, Index.

Editorial Reviews

"...the sources used are authoritative, and the overall execution excellent. Anyone working with this type of cell culture would find this volume a highly valuable resource. The future volumes of this excellent series will be eagerly anticipated." Sara Abraham, Canadian Journal of Medical Laboratory Science

From the Publisher