Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas: Molecular Genetics; Lung and Breast Carcinomas

by M. A. Hayat (Editor)

Overview

The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.

Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.

• The only book available that translates molecular genetics into cancer diagnosis
• Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
• Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
• Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
• Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
• Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
• Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References

Product Details

ISBN-13:	9780080495194
Publisher:	Elsevier Science
Publication date:	06/16/2004
Sold by:	Barnes & Noble
Format:	eBook
Pages:	556
File size:	25 MB
Note:	This product may take a few minutes to download.

About the Author

Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

Table of Contents

Comparison of Immunohistochemistry, in Situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ Hybridization
Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization and Immunohistochemistry
Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization
Polymerase Chain Reaction
DNA Microarrays Technology
Tissue Microarrays and their Modifications in Highthoroughput Analysis of Clinical Specimens
Gene Expression Profiling Using Laser Microdissection in Cancer Tissues
Differential Display of Gene Expression in Human Carcinomas
Serial Analysis of Gene Expression in Human Diseases
Lung Carcinoma: An Introduction
Hitopathological Classification and Phenotype of Lung Tumors
Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma
Immunohistochemical Expression of MDM2 in Lung Cancer
Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma
Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma
Immunohistochemistry of Laminin-5 in Lung Carcinoma
Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma
Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma
Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma
Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma
In Situ Hybridization and Immunohistochemistry of Telomerase in Lung Carcinoma
Use of Fluorescence in situ Hybridization in Detection of Lung Cancer Cells
Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma
Breast Carcinoma: An Introduction
Expression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/KDR in Breast Carcinoma
HER2/Neu Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and Fluorescence in situ Hybridization
Her-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples from Breast Cancer
Detection of HER-2 Oncogene with Chromogenic in situ Hybridization in Breast Carcinoma
Immunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma Patients
CD10 Expression in Normal Breast and Breast Cancer Tissue
Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma
Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma
Immunohistochemistry of Adhesion Moleculae Ceacam1 Expression in Breast Carcinoma
Role of Cadherins in Breast Cancer
Immunohistochemical Expression of Erythropoitein and Erythropoitin Receptor in Breast Carcinoma
Loss of BRCA1 Expression in Breast Carcinoma
Role of Immunohistochemical Expression of BRCA1 in Breast Cancer
Fluorescence in situ Hybridization of BRCA1 Gene in Breast Carcinoma
Immunohistochemistry of C-MYC Expression in Breast Carcinoma
Immunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma: A Possible Molecular Marker for Diagnosis
Role of the Epidermal Growth Factor Receptor in Breast Carcinoma
Alterations of the Cell Cycle Regulating Proteins in Invasive Breast Cancer
Correlation with Proliferation, Apoptosis, and Clinical Outcome
Immunohistochemistry of Estrogen Receptor Expression in Breast Carcinoma
Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Carcinoma
Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma
Immunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast Carcinoma
Role of Immunohistochemical Expression of Receptors in Male Breast Carcinoma
Detection of Glycoconjugates in Breast Cancer Cell Lines: Confocal Fluorescence
Role of ETV6-NTRK3 Gene Fusion in Breast Carcinoma
Role of CA6 Protein Expression in Breast Carcinoma
Immunohistochemistry of Effusions
Immunohistochemistry of Needle Cytopunctures of Breast Carcinomas

What People are Saying About This

From the Publisher

Presents the latest in in situ hybridization and immunohistochemistry techniques expected to become an important part of cancer diagnosis in clinical practice in the future.

Editorial Reviews

3 Stars from Doody

Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: This is a handbook of primarily histochemical methods applied to diagnosis of lung and breast carcinomas. Protocols are included.
Purpose: The goal is to offer a set of protocols for common histochemical methods used in tumor diagnosis. This volume focuses on lung and breast; two additional volumes are planned. It may be of utility to some practicing pathologists (most will have comparable recipes to hand). It certainly will be useful to someone not familiar with the techniques.
Audience: The intended audience is the pathologist engaged in diagnostic work. The editor has assembled a large and diverse group of international contributors; the authority level varies.
Features: Early diagnosis of malignant disease remains the best route to effective therapy. The classic methods of histochemical analysis still form the backbone of most diagnostic procedures. This is the first of three planned volumes on these methods of tumor diagnosis; the focus is on lung and breast carcinomas. Each major section contains several chapters that provide detailed protocols for (mainly) histochemical identification of specific molecules associated with the given malignancy. Additional coverage is provided for array methods. The technical information is clearly of value to clinical pathologists although it is likely that most practitioners have already established methods. As opposed to general visual examination of biopsy sections, the emphasis here is on proteins known to be disease related. There are ample, well produced illustrations and suitable references for each chapter. A minor concern is that the international author cadre would have profited (linguistically) from more careful editing. Some of the chapter titles promise more than they deliver and there is insufficient attention given to DNA-based approaches.
Assessment: These caveats aside, the volume has much practical information and would serve well in most clinical pathology laboratories. It is likely that many of these procedures will be superseded by DNA-based methods but these are still some time away.

Doody's Review Service

"...this handbook is of unparalleled value as a reference book and should be on the shelf of all serious investigators in the field... Its value lies in having an immediate reference source at hand, replete with bibliography, for a particular research project, or occasionally for a particularly difficult clinical issue. In these areas, there are simply no competitors in print..." —Clive R. Taylor, Dept of Pathology, The Keck School of Medicine at the University of Southern California, Los Angeles, in APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY (June 2006)

"...this book is a valuable reference for medical students, medical technician, pathologists, and clinician alike who are interested in tumor biology or translational research." —HUMAN PATHOLOGY (July 2005)

"Volume 1 of this three-volume Elsevier handbook, edited by M.A. Hayat on the 'Immunohistochemistry and in situ Hybridization of Human Carcinomas' is impressive. ...The technical detail included in many contributions surpasses that available from the scientific and clinical journals; all provide a depth of up-to-date knowledge based upon the authors' experience and expertise. Every large hospital and medical school library should be persuaded to buy a copy of this book." —MICRON (2005)

"...Professor Hayat’s handbook, Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas has the great potential of becoming the handbook of choice for clinician and basic scientists including medical and biological students and biomedical technologists." —Akhouri A. Sinha, Ph.D., Professor of Genetics, Cell Biology and Development, University of Minnesota and Research Scientist at the VA Medical Center
Minneapolis, MN

From the Publisher