Hematologic Malignancies: Methods and Techniques / Edition 1

by Guy B. Faguet

Overview

The aim of Hematologic Malignancies: Methods and Techniques is to review those methods most useful for the diagnosis and subsequent mana- ment of hematologic malignancies. The scope of coverage is intentionally broad, ranging from routine procedures to highly sophisticated methods not currently offered by most clinical laboratories. The latter methods were selected especially to bring into focus recent advances in molecular biology that, since they provide us with strong tools for assessing the outcome of upcoming therapeutic modalities intent on disease eradication, are expected to impact the future diagnosis and management of these diseases. Thus, the c- mon thread among all chapters is clinical relevance, whether sanctioned by past experience or by the expectation that seemingly esoteric research techniques of today will prove clinically valuable in the future. Hematologic Malignancies: Methods and Techniques is primarily a compilation of methods presented in sufficient detail—by authors with extensive expertise in their field—to serve not only as a reference for seasoned research and clinical laboratory pers- nel, but also as a guide for the less experienced. Moreover, the contributing authors also discuss the pathophysiologic bases and the diagnostic usefulness that underscore each method’s clinical relevance. Thus, this volume should be also valuable to clinicians—especially hematologists, oncologists, and path- ogists—often bewildered by an ever increasing flow of new scientific inf- mation, the practical application of which is often either not clearly disclosed or difficult to discern.

Product Details

ISBN-13:	9780896035430
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/30/2000
Series:	Methods in Molecular Medicine , #55
Edition description:	2001
Pages:	360
Product dimensions:	5.98(w) x 9.02(h) x 0.04(d)

About the Author

Faguet, Guy B. (Medical Coll of Georgia)

Table of Contents

Cytogenetics.- Cytogenetics Analysis.- FISH Analysis.- Comparative Genomic Hybridization for the Analysis of Leukemias and Lymphomas.- Spectral Karyotyping (SKY) of Hematologic Malignancies.- Polymerase Chain Reaction.- Molecular Genetic Methods in Diagnosis and Treatment.- Detecting Monoclonal Immunoglobulin andT-Cell Receptor Gene Rearrangements in B- andT-Cell Malignancies by Polymerase Chain Reaction.- Quantifying Residual Leukemia by “Clone-Specific” Polymerase Chain Reaction.- NPM-ALK Reverse Transcriptase-Polymerase Chain Reaction for Detecting the t(2;5) of Non-Hodgkin’s Lymphoma.- Flow Cytometry.- Diagnostic Flow Cytometry in Hematologic Malignancies.- Flow Cytometric Analysis of Hematologic Neoplasia.- In Situ Hybridization in Flow Cytometry.- Quantitative Fluorescence Cytometry.- Special Histopathology Stains.- Methods in Leukocyte Cyhemistry.- Immunohishemistry in Leukemias and Lymphomas.- Molecular Genetics and Cytokines.- Apoptosis.- Cytokine Receptors.

Editorial Reviews

Reviews many of the key molecular methods used for the diagnosis and management of hematologic malignancies. These clinically relevant techniques range from routine test procedures to methods offered only by specialized reference laboratories, and fall into five major groups: cytogenics, polymerase chain reaction, flow cytometry, cytochemistry and immunochemistry, and apoptosis and cytokine receptors. Discusses the rationale, interpretation, and clinical relevance of each method, and describes each method in step-by-step detail. Of interest to hematologists, oncologists, and pathologists. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

"Serving both clinical and experimental needs, Hematologic Malignancies: Methods and Techniques, provides an array of powerful tools that will guide clinicians - especially hematologists, oncologists, and pathologists - to better diagnose and manage their patients with hematologic malignancies, and enable researchers to assess the anticancer effect of agents that impact cancer cells at the molecular level." -Tumori

"Guy B. Faguet, as editor, and the selected number of experts that he gathered together for this book, have done a remarkable job. In fact, there are very few books that in so few pages contain as much, and as useful, information as this newcomer in the field. All in all...this is an extremely valuable book of particular use to students and trainees in hematology and oncology." -Annals of Oncology

From the Publisher