Hepatocytes: Methods and Protocols

Hepatocytes: Methods and Protocols

Hepatocytes: Methods and Protocols

Hepatocytes: Methods and Protocols

Paperback(1st ed. 2022)

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Overview

This volume details experimental prools to study structure and function of hepaytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepaytes in healthy and in injured liver. Chapters guide readers through separating hepayte subfractions, characterizing the heterogeneity, novel culture systems, hepayte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepaellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible prools.

Authoritative and cutting-edge, Hepaytes: Methods and Prools aims to provide techniques to further understand molecular mechanisms involved in the regulating hepayte functions and underlying liver diseases.


Product Details

ISBN-13: 9781071625590
Publisher: Springer US
Publication date: 09/22/2023
Series: Methods in Molecular Biology , #2544
Edition description: 1st ed. 2022
Pages: 281
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Long-Term Expansion of Primary Hepayte Organoids.- Generation of in vivo traceable hepayte-like cells from human iPSCs.- Generation of hepayte organoids from human iPS cells.- Induction of bile canaliculi forming hepaytes from human induced pluripotent stem cells.- Human hepayte transduction with adeno-associated virus vector.- Hepayte-directed delivery of Lipid-encapsulated small interfering RNA.- Co-culture model for hepatitis B virus infection using iPSC-derived liver progenitor cells and liver sinusoidal endothelial cells.- In vitro assay system to detect drug-induced bile acid-dependent cytotoxicity using hepaytes.- Evaluation of urea cycle activity by metabolic flux analysis using mass spectrometry.- Live Cell Imaging of Biosynthetic Protein Transport in Hepaytes.- Collagen sandwich culture of primary hepaytes for image-based investigations.-Assessment of Hepayte Ploidy by Flow Cytometry.- Isolation of small hepayte-like progenitor cells from retrorsine/partial hepatectomy rat livers by laser micro-dissection.- Application of EdU-based DNA synthesis assay to measure hepayte proliferation in situ during liver regeneration.- Quantification of clonal expansion of hepaytes in normal and injured liver.- Evaluation of SOX9-positive hepaytes in human liver specimens and mature mouse hepaytes.- Identification of genes regulating hepayte injury by a genome-wide CRISPR-Cas9 Screen.- Identification of subtypes of HCC using bioinformatics and the hepayte differentiation model.- 3D culture of primary patient-derived hepatoblastoma tumoroids.- Preparation of functional heapytes.

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