High Content Screening / Edition 1

by D. Lansing Taylor

Overview

There has always been some tension between proponents of hypothesis-driven and discovery-driven research in the broad field of life sciences. Academic research has been primarily focused on hypothesis-driven research. However, the success of the human genome project, a discovery-driven research approach, has opened the door to adding other types of discovery-driven research to a continuum of research approaches. In contrast, drug discovery research in the pharmaceutical industry has embraced discovery-driven research for many years. A good example has been the discovery of active compounds from large chemical libraries, through screening campaigns. The success of the human genome project has also demonstrated the need for both academic researchers and industrial researchers to now understand the functions of genes and gene products. The cell is the basic unit of life and it has been at the cellular level where function can be demonstrated most cost-effectively and rapidly. High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell.

Product Details

ISBN-13:	9781588297310
Publisher:	Springer-Verlag New York, LLC
Publication date:	08/15/2006
Series:	Methods in Molecular Biology , #356
Edition description:	2006
Pages:	464
Product dimensions:	7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

to High Content Screening.- Past, Present, and Future of High Content Screening and the Field of Cellomics.- A Pharmaceutical Company User’s Perspective on the Potential of High Content Screening in Drug Discovery.- Linking Microscopy and High Content Screening in Large-Scale Biomedical Research.- Instrumentation, Biological Application Software, and Sample Preparation.- Requirements, Features, and Performance of High Content Screening Platforms.- Characteristics and Value of Directed Algorithms in High Content Screening.- Characteristics and Value of Machine Learning for Imaging in High Content Screening.- Tools for Quantitative and Validated Measurements of Cells.- Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays.- Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications.- Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel.- Reagents.- Reagents to Measure and Manipulate Cell Functions.- Fluorescent Proteins and Engineered Cell Lines.- Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays.- The HaloTag™.- Protein Labeling With FlAsH and ReAsH.- Exploiting Network Biology to Improve Drug Discovery.- Physiological Indicators of Cell Function.- The Use of siRNA to Validate Immunofluorescence Studies.- Caged Substrates Applied to High Content Screening.- Informatics and Bioinformatics.- Overview of Informatics for High Content Screening.- Large-Scale Data Management for High Content Screening.- An Integrated Biomedical Knowledge Extraction and Analysis Platform.- Visualization of High Content Screening Data.- Pathway Mapping Tools for Analysis of High Content Data.- Assays and Applications of High Content Screening.- SystemsBiology in Cancer Research.- Target Validation in Drug Discovery.- High Content Screening as a Screening Tool in Drug Discovery.- Discovery of Protein Kinase Phosphatase Inhibitors.- High Content Translocation Assays for Pathway Profiling.- In Vitro Cytotoxicity Assessment.- Neurite Outgrowth in Retinal Ganglion Cell Culture.

Editorial Reviews

Reviewer: Scott J Weir, PharmD, PhD (University of Kansas Medical Center)
Description: This book describes the evolution and current state of high content screening (HCS).
Purpose: The purpose is to describe the history of HCS; the instrumentation, software applications and sample preparation involved in HCS; markers employed (they refer to reagents); bioinformatics practices and requirements; and examples of applications in cell biology and drug discovery. This book is applicable to current cell biology and drug discovery research. With the availability of siRNA libraries for commercial purchase by universities and industry, this book is very timely.
Audience: It is intended for researchers currently working in HCS or considering HCS as a research tool, whether in academia or industry. The authors, as a rule, are very credible. It is clear that care was taken to select authors who were, or are currently, very active in the field of HCS.
Features: The chapter by Dr Taylor on the history of HCS is very helpful. I particularly liked the sections addressing bioinformatics needs and the application examples. It would be wise, in my opinion, to plan on a second edition perhaps in three years, due to the rapidly changing HCS field.
Assessment: This is one of the better books I have reviewed. As someone who works in drug discovery and early drug development, I found the book very useful.

Doody's Review Service

"...very useful." -Weighted Numberical Score: 97 - 5 Stars!-Doody's Health Science Book Review Journal

From the Publisher