High Performance Liquid Chromatography

by Heinz Engelhardt, G. Gutnikov (Translator)

Overview

Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. If the statisticians are to be believed, the recent growth of LC has been the most specta­ cular development in analytical chemistry and has not yet abated because its vast potential for application remains to be fully exploit­ ed. Significant factors contributing to this continued rise are the simplicity and low cost of the required basic equipment and the rela­ tive ease of acquiring and interpreting the data. Unfortunately, in LC, as so often in the field of analytical chemistry, the available commercial instruments are frequently far more complicated - and consequently far more expensive - than is nec­ essary for routine application. Therein also lies the risk of propa­ gating a "black box" philosophy that would be particularly detrimen­ tal to chromatography. Moreover, it appears to have been forgotten, as was done previously with gas chromatography, that inadequate sep­ aration by a column can be remedied only with great difficulty, if at all, by electronic means. Also, whether the capillary columns recent­ ly advocated with great enthusiasm for LC will fulfill the expecta­ tions of their proponents is highly questionable unless someone comes up with some new and revolutionary ideas.

Product Details

ISBN-13:	9783642670664
Publisher:	Springer Berlin Heidelberg
Publication date:	01/19/2012
Series:	Chemical Laboratory Practice
Edition description:	Softcover reprint of the original 1st ed. 1979
Pages:	254
Product dimensions:	6.69(w) x 9.61(h) x 0.02(d)

Table of Contents

I. Chromatographic Processes.- References Chapter I.- II. Fundamentals of Chromatography.- A. Retention.- B. Linear Flow Rate, Porosity, Permeability.- C. Band Broadening.- D. Resolution.- E. Dependence of Band Broadening on Flow Rate.- F. Band Broadening and Particle Size.- G. Extra-Column Band Broadening.- H. Optimum Analysis Conditions and Analysis Time.- I. Selection of a Suitable Column.- References Chapter II.- III. Equipment for HPLC.- A. Solvent Reservoir — Degassing of the Eluent.- B. Pumps.- C. Damping of the Pulsations.- D. Sample Introduction.- E. The Column.- F. Thermostating.- G. Measurement of the Flow Rate.- H. Fraction Collectors.- I. Recorders.- J. Instrumentation for Gradient Elution.- K. Safety Measures.- References Chapter III.- IV. Detectors.- A. UV Detectors.- B. Differential Refractometer.- C. Microadsorption Detector.- D. Transport Detector (Flame Ionization Detector).- E. Fluorescence Detector.- F. Other Detectors.- G. Comparison of the Important Detectors.- H. Reaction Detectors.- References Chapter IV.- V. Stationary Phases.- A. Packing Materials for Adsorption and Partition Chromatography.- B. Chemically Modified Supports.- C. Ion Exchangers.- D. Stationary Phases for Exclusion Chromatography.- References Chapter V.- VI. Adsorption Chromatography.- I. Polar Stationary Phases.- II. Nonpolar Stationary Phases.- III. The General Elution Problem.- IV. Applications of Adsorption Chromatography.- References Chapter VI.- VII. Partition Chromatography.- A. Introduction.- B. Supports and Liquid Phases.- C. Properties of the Column.- D. Applications.- E. Ion-pair Chromatography.- References Chapter VII.- VIII. Ion-Exchange Chromatography.- A. Principle.- B. Ion Exchange Materials.- C. Characterization of the Ion Exchangers.- D. Optimizing aSeparation.- E. Applications.- References Chapter VIII.- IX. Exclusion Chromatography. Gel Permeation Chromatography.- A. Introduction.- B. Basics of Exclusion Chromatography.- C. Stationary Phases for Exclusion Chromatography.- D. Applications of Exclusion Chromatography.- References Chapter IX.- X. Selection of the Separation System.- References Chapter X.- XI. Special Techniques.- A. Preparative Chromatography.- B. Qualitative Analysis.- C. Quantitative Analysis.- D. Trace Analysis.- References Chapter XI.- XII. Purification of Solvents.- References Chapter XII.