High Resolution Separation and Analysis of Biological Macromolecules, Part A: Fundamentals / Edition 1

by John N. Abelson, Melvin I. Simon, Barry L. Karger, William Hancock

Overview

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today—truly an essential publication for researchers in all fields of life sciences.

Product Details

ISBN-13:	9780121821715
Publisher:	Elsevier Science
Publication date:	06/24/1996
Series:	Methods in Enzymology , #270
Pages:	622
Product dimensions:	6.00(w) x 9.00(h) x (d)

About the Author

Karger, Barry L. (Northeastern Univ); Hancock, William S. (Hewlett Packard Company)

The contributors represent the specialties of chemistry, biochemistry, chemical engineering, pharmacology, biology, and analytic chemistry. Most are from universities and research institutes in the U.S., Australia, Czech Republic, Italy, Canada, and Japan. Institutions prominently represented include Monash Univ, Institute of Analytical Chemistry, Univ of North Carolina, Univ of Ottawa, and Himeji Institute of Technology.

Table of Contents

Liquid Chromatography: Methods: M.-I. Aguilar and M.T.W. Hearn, High-Resolution Reversed-Phase High-Performance Liquid Chromatography of Peptides and Proteins. S.-L. Wu and B.L. Karger, Hydrophobic Interaction Chromatography of Proteins. G. Choudhary and C. Horvath, Ion-Exchange Chromatography. M. de Frutos, S.K. Paliwal, and F.E. Regnier, Analytical Immunology. Columns and Instrumentation: M.V. Novotny, Microcolumn Liquid Chromatography in Biochemical Analysis. S.K. Paliwal, M. de Frutos, and F.E. Regnier, Rapid Separations of Proteins by Liquid Chromatography. L.R. Snyder, Automated Method Development in High-Performance Liquid Chromatography. M. Szulc, R. Mhatre, J. Mazzeo, and I.S. Krull, Detection in Liquid Chromatography. G.P. Rozing, Diode Array Detection. Electrophoresis: Slab Gel Electrophoresis: High Resolution: P.G. Righetti, C. Gelfi, and M. Chiari, Isoelectric Focusing in Immobilized pH Gradients. J.C. Wrestler, B.D. Lipes, B.W. Birren, and E. Lai, Pulsed-Field Gel Electrophoresis. G.W. Slater, P. Mayer, and G. Drouin, Migration of DNA through Gels. Capillary Electrophoresis: S. Hjerten, Capillary Electrophoretic Separation in Open and Coated Tubes. N. Matsubara and S. Terabe, Micellar Electrokinetic Chromatography. S. Micinski, M. Gronvald, and B.J. Compton, Structure–Mobility Relationships in Free Solution Zone Electrophoresis. T. Wehr, M. Zhu, and R. Rodriguez-Diaz, Capillary Isoelectric Focusing. L. Krivankova[breve over r], P. Gebauer, and P. Bocek[breve over c], Isotachophoresis. A.W. Moore, Jr., J.P. Larmann, Jr., A.V. Lemmo, and J.W. Jorgenson, Two-Dimensional Liquid Chromatography–Capillary Electrophoresis Techniques for Analysis of Proteins and Peptides. T.T. Lee and E.S. Yeung, Capillary Electrophoresis Detectors: Lasers. Mass Spectrometry: W.E. Seifert, Jr., and R.M. Caprioli, Fast Atom Bombardment Mass Spectrometry. F. Banks, Jr., and C.M. Whitehouse, Electrospray Ionization Mass Spectrometry. R.C. Beavis and B.T. Chait, Matrix-Assisted Laser Desorption Mass–Spectrometry of Proteins. J.C. Schwartz andI. Jardine, Quadrupole Ion Trap Mass Spectrometry. Author Index. Subject Index.

Editorial Reviews

Praise for the Volume
"These two books comply with and add to the outstanding reputation of the Methods in Enzymology series as a primary source for detailed and practical information on techniques used in the biological sciences. The excellent, step-by-step description of the techniques described, the clear schematic illustrations, and the frequent addition of technical tricks... will facilitate the successful application of the techniques." —DANIEL FIGEYS and RUEDI AEBERSOLD

Praise for the Series
"The Methods in Enzymology series represents the gold-standard." —NEUROSCIENCE

"Incomparably useful." —ANALYTICAL BIOCHEMISTRY

"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." —BIO/TECHNOLOGY

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." —CHEMISTRY IN INDUSTRY

"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." —AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." —ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." —JOURNAL OF CHROMATOGRAPHY

From the Publisher