Lactoferrin: Interactions and Biological Functions / Edition 1

by T. William Hutchens, Bo Lönnerdal

Overview

The number of investigators focusing their attention on lactoferrin has increased dramatically in recent years. Lactoferrin is a protein with more than one known structure and a number of proposed biological functions, including several with important regulatory consequences. In many ways it has been an easy protein to investigate; however, there have been difficulties understanding specific structure / function relationships, particularly as it functions in vivo. Research funding dedicated to this protein has previously been limited, but is now increasing. As lactoferrin begins to emerge formally as a protein of significance to the medi­ and industry, it is more important than ever to coor­ cal profession dinate and integrate research efforts whenever possible and to share the results of these efforts within the expanding array of medical and scientific diSciplines involved. It was our intention to provide a forum to summarize and disseminate the most recent advances in this field. Included in Lactoferrin: Interactions and Biological Functions are selected presentations representing the many disciplines involved in defining lactoferrin function in terms of its known structural features, including its carbohydrate side-chains, receptor binding sites, its capacity to bind different metal ions, and other newly discovered bioactive domains. Several of the possible physiologi­ cal functions of lactoferrin are described and summarized in detail, including the role of laetoferrin in bacterial killing, its in­ volvement in cell growth and proliferation, in the modulation of immune function, and in iron absorption.

Product Details

ISBN-13:	9780896033665
Publisher:	Springer-Verlag New York, LLC
Publication date:	03/24/1997
Series:	Experimental Biology and Medicine , #28
Edition description:	1997
Pages:	408
Product dimensions:	5.98(w) x 9.02(h) x 0.04(d)

About the Author

Hutchens, T. William (UC Davis); Lonnerdal, Bo (UC Davis)

The contributors represent the specialties of biochemistry, chemistry, biology, nutrition science, genetics, and infectious diseases. Most are from universities and research institutes in the U.S., Canada, Italy, France, New Zealand, and Japan. Institutions prominently represented include Massey Univ, New York Univ, Mount Sinai School of Medicine, Univ des Sciences et Technologies de Lille, Univ of Iowa, Baylor, UC Davis, Univ of Genoa, and Univ of Calgary.

Table of Contents

I: Lactoferrin Structure and Function.- 1. Lactoferrin Structure-Function Relationships: An Overview.- 2. Altered Domain Closure and Iron Binding in Lactoferrin Mutants.- 3. Affinity Mass Spectrometry: Probes with Surfaces Enhanced for Affinity Capture (SEAC) of Lactoferrin.- II: Lactoferrin Gene Expression.- 4. Variants and Biotechnological Use of the Bovine Lactoferrin-Encoding Gene.- 5. Posttranscriptional Regulation of Bovine and Human Lactoferrin: Species Differences and Influence of mRNA Regions.- 6. Mutagenesis of Human Lactoferrin and Expression in Baby Hamster Kidney Cells.- 7. Structural Determination of Two N-Linked Glycans Isolated from Recombinant Human Lactoferrin Expressed in BHK Cells.- 8. Distribution of the Iron-Binding Protein Lactof errin in the Pathological Lesions of Neurodegenerative Diseases.- 9. Lactoferrin Almost Absent from Lactating Rat Mammary Gland Is Replaced by Transferrin.- 10. Estrogen Regulation of Human Lactoferrin Gene Activity: Transcriptional Synergism Between Estrogen Receptorand Related Orphan Receptor.- 11. Expression and Functional Analysis of Recombinant Human Lactoferrin.- 12. Structural and Functional Flexibility of Lactoferrin.- 13. Difference in Binding and Fate of Lactotransferrin in Jurkat Human Lymphoblastic T-Cells and in T-47D Human Breast Cancer Cells.- 14. The Impact of Phagocyte-Lactoferrin Interactions on Inflammation.- 15. Regulation of Lymphocyte Proliferation by Lactoferrin.- 16. Recombinant Human Lactoferrin and Its Variants: Receptor Binding in Human Intestinal Brush-Border Membranes.- III: Functions Related to Lactoferrin Interactions with Prokaryotic and Eukaryotic Cells.- 17. Influence of Lactoferrin on Host—Microbe Interactions.- 18. Bacterial Lactoferrin Receptors in the Neisseriaceae.- 19.Bacteriostatic Effects of Orally Administered Bovine Lactoferrin on Intestinal Bacteria in the Gut of Mice Fed Bovine Milk.- 20. Functional Significance of the Binding of Lactoferrin to DNA.- 21. Specific Binding of Ferrilactoferrin and Ferritransf errin in the Protozoan Leishmania chagasi.- IV: Lactoferrin Metabolism.- 22. Observations on the Metabolism and Cellular Interactions of Lactoferrin.- 23. Lactoferrin as a Possible Transcriptional Regulator: Downmodulation of the Granulocyte-Macrophage Colony-Stimulating Factor Promoter.- V: The Use of Lactoferrin as a Food Additive.- 24. Modification of Infant Formula: The Case of Lactoferrin.- 25. Lactoferrin in Infant Formulas: How and Why?.

Editorial Reviews

Reviewer: Richard S. Eisenstein, AB, PhD (University of Wisconsin-Madison)
Description: This book deals with many aspects of the biology of the interesting but incompletely understood iron binding protein, lactoferrin. Much remains to be learned about the function of lactoferrin in health and disease, and this book does an admirable job of describing lactoferrin function at the organismal, cellular, and molecular levels.
Purpose: The purpose is to describe the current state of knowledge regarding lactoferrin structure, function, and the cellular roles of this iron binding protein. An important aspect of lactoferrin function appears to be its effect on bacterial growth in normal and diseased states and excellent coverage of this area is provided. Furthermore, some theories of lactoferrin function are not widely accepted, and the book identifies what needs to be done to more fully understand the lactoferrin function.
Audience: The audience includes nutritionists, biochemists, animal scientists, and those interested in the molecular physiology of lactoferrin. Included among this group are physicians interested in the apparent roles of lactoferrin in infant nutrition/health as well as in the potential role of lactoferrin in disease.
Features: The book is comprehensive. Lactoferrin biology is discussed in five parts: the structure and function of lactoferrin; lactoferrin gene expression and manipulation of the lactoferrin gene for biotechnological purposes; functional effects of lactoferrin interactions with eukaryotic and prokaryotic cells; lactoferrin metabolism; and lactoferrin as a food additive.
Assessment: This collection of articles on lactoferrin, which includes chapters by many leaders in the field, will be of interest and significance to those interested in specific aspects of infant nutrition as well as the metabolism of iron in health and disease. Biochemists and molecular biologists interested in the structure and expression of this mammalian iron binding protein will also find this book of significant use.

Doody's Review Service

3 Stars from Doody

This book deals with many aspects of the biology of the interesting but incompletely understood iron binding protein, lactoferrin. Much remains to be learned about the function of lactoferrin in health and disease, and this book does an admirable job of describing lactoferrin function at the organismal, cellular, and molecular levels. The purpose is to describe the current state of knowledge regarding lactoferrin structure, function, and the cellular roles of this iron binding protein. An important aspect of lactoferrin function appears to be its effect on bacterial growth in normal and diseased states and excellent coverage of this area is provided. Furthermore, some theories of lactoferrin function are not widely accepted, and the book identifies what needs to be done to more fully understand the lactoferrin function. The audience includes nutritionists, biochemists, animal scientists, and those interested in the molecular physiology of lactoferrin. Included among this group are physicians interested in the apparent roles of lactoferrin in infant nutrition/health as well as in the potential role of lactoferrin in disease. The book is comprehensive. Lactoferrin biology is discussed in five parts: the structure and function of lactoferrin; lactoferrin gene expression and manipulation of the lactoferrin gene for biotechnological purposes; functional effects of lactoferrin interactions with eukaryotic and prokaryotic cells; lactoferrin metabolism; and lactoferrin as a food additive. This collection of articles on lactoferrin, which includes chapters by many leaders in the field, will be of interest and significance to those interested in specific aspects of infant nutrition as well asthe metabolism of iron in health and disease. Biochemists and molecular biologists interested in the structure and expression of this mammalian iron binding protein will also find this book of significant use.

Richard S. Eisenstein

Twenty-five contributions, representing both experimental and clinical investigators, explain our current understanding of lactoferrin. The authors define the structural features of lactoferrin and describe several of its physiological functions, including its role in killing bacteria, and its involvement in cell growth and proliferation, the modulation of immune function, and iron absorption. Particular attention is given to lactoferrin functions in the regulation of normal and diseased states. New knowledge of the molecular biology of lactoferrin is also included, along with discussions of commercially viable large-scale production techniques and new industrial applications. Annotation c. by Book News, Inc., Portland, Or.

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