Mammalian and Avian Transgenesis - New Approaches / Edition 1

by Shirley Pease, Carlos Lois

Overview

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains prools for successful transgenesis in rats, cows, pigs and birds.

The manual provides detailed, step-by-step prools covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Product Details

ISBN-13:	9783540284154
Publisher:	Springer Berlin Heidelberg
Publication date:	12/21/2005
Series:	Principles and Practice
Edition description:	2006
Pages:	281
Product dimensions:	6.10(w) x 9.25(h) x (d)

Table of Contents

Generation of Transgenic Animals Using Lentiviral Vectors.- Intracytoplasmic Sperm Injection (ICSI) in the Mouse.- Generation of Embryonic Stem (ES) Cell-Derived Embryos and Mice by Tetraploid-Embryo Complementation.- Cloning The Laboratory Mouse by Nuclear Transfer.- Large Insert Transgenesis.- Regional and Temporal Control of Genetic Manipulation in the Mouse.- High Resolution Gene Expression Analysis in Mice Using Genetically Inserted Reporter Genes.- Nuclear Transfer in the Cow.- Production of Transgenic Pigs by DNA Microinjection.- Production of Transgenic Birds Using Lentiviral Vectors.- Ancillary Techniques.