Manual of Sperm Retrieval and Preparation in Human Assisted Reproduction

by Ashok Agarwal (Editor), Ahmad Majzoub (Editor), Sandro C. Esteves (Editor)

Overview

Obtaining good quality sperm for in-vitro fertilization, ICSI, or for cryopreservation can be a major problem if a man suffers from medical conditions that preclude normal collection. Major advances in sperm retrieval and preparation techniques have been witnessed in recent years, allowing a better understanding of the contribution of the sperm to a couple's fertility. This Manual is a complete guide to sperm retrieval methods performed for men with azoospermia. Covering all the basic and advanced steps for implementation of these procedures, the Manual includes descriptions of the underlying science along with detailed, practical advice on methods, including tips for optimizing outcomes and trouble-shooting, using an evidence-based approach. This will be an invaluable resource for andrologists, urologists, embryologists, male fertility specialists, gynecologists, and other healthcare workers practising reproductive medicine. It is particularly valuable for reproductive laboratory personnel wishing to refine or develop technique and improve outcomes, and for IVF Quality Managers.

Product Details

ISBN-13:	9781108856768
Publisher:	Cambridge University Press
Publication date:	06/10/2021
Sold by:	Barnes & Noble
Format:	eBook
File size:	15 MB
Note:	This product may take a few minutes to download.

About the Author

Ashok Agarwal is the Director of the Clinical Andrology Laboratory and Reproductive Tissue Bank and the Director of Research at the American Center for Reproductive Medicine. He holds these positions at The Cleveland Clinic Foundation, where he is a Professor at the Lerner College of Medicine of Case Western Reserve University, Cleveland, Ohio, USA.
Ahmad Majzoub is a Consultant in the Department of Urology at the Hamad Medical Corporation, Doha and an Assistant Professor of Clinical Urology at Weill Cornell Medicine, Doha, Qatar.
Sandro C. Esteves is Medical Director, ANDROFERT Andrology & Human Reproduction Clinic, Campinas, and a Professor of Urology at the University of Campinas (UNICAMP), Campinas, Brazil.

Table of Contents

Part I. Introduction: 1. Anatomy and physiology of the male reproductive system Ryan Scribner and Nick N. Tadros; 2. Evaluation of candidates for sperm retrieval Darren J. Bryk, Neel V. Parekh and Sarah C. Vij; 3. Testicular histopathology and the role of testis biopsy Gianmartin Cito; Part II. Sperm Retrieval: 4. History of surgical sperm retrieval techniques Rupin Shah; 5. Epididymal sperm retrieval: indications, surgical protocol, and outcomes Jason P. Akerman and R. Matthew Coward; 6. Testicular sperm retrieval: indications, surgical protocol, and outcomes Ahmad Majzoub, Sandro C. Esteves and Chak-Lam Cho; 7. Surgical and non-surgical sperm retrieval techniques in patients with ejaculatory dysfunctions Mohamed Adnan, Omer Raheem and Laith M. Alzweri; 8. Sperm retrieval in non-azoospermic men: indications, protocol, and outcomes Sandro C. Esteves and Ahmad Majzoub; 9. Predictors of positive surgical sperm retrieval in azoospermic males: evaluation of clinical, laboratory, and histopathologic factors Kareim Khalafalla, Ahmed H. Almalki, Ahmad Majzoub and Sandro C. Esteves; 10. Methods for enhancing surgical sperm retrieval success rate Jordan Cohen and Ranjith Ramasamy; Part III. Laboratory Handling of Retrieved Sperm: 11. Critical factors for optimizing sperm handling and ICSI outcomes Edson Borges, Amanda Souza Setti and Daniela Paes de Almeida Ferreira Braga; 12. Sperm cryopreservation Rakesh Sharma, Kruyanshi Master and Ashok Agarwal; 13. Future directions in sperm handling for ART Manesh Panner Selvam Kumar, Catalina Barbarosie and Ashok Agarwal.

Editorial Reviews

Reviewer: Irvin H Hirsch, MD (Thomas Jefferson University)
Description: Advances in assisted reproductive technology (ART) over the past three decades have helped severely infertile couples achieve biologic paternity. This book offers an excellent resource to understanding the pathophysiologic basis for sperm acquisition in the clinical settings of ablative and obstructive azoospermia and in ejaculatory dysfunction.
Purpose: The laboratory progress achieved in in-vitro fertilization has been accompanied by advances in the surgical treatment of azoospermic men, particularly the refinement of sperm quality utilized in these assisted laboratory settings. This manual presents a concise but comprehensive compendium of the management of patients requiring sperm retrieval and contemporary studies reviewing predictors of successful ART outcomes.
Audience: The authors and contributors are world-renowned leaders in the fields of andrology, human reproduction, and urology. They target an interested audience that includes reproductive endocrinologists, urologists, and embryologists with an encompassing presentation of the topic of sperm retrieval techniques.
Features: As a prelude to providing the practical details of various sperm retrieval methodologies, this manual opens with normal male reproductive anatomy and physiology and clinically relevant aspects of severe male reproductive dysfunction: obstructive and non-obstructive azoospermia and ejaculatory dysfunction. The authors dedicate much of their effort to surgical techniques of sperm retrieval from the epididymis and testis with clear step-by-step explanation. They also present methods of ejaculatory stimulation in men with dysfunctional ejaculation. These methods are compared and tailored to specific clinical indications. Regardless of the technique of choice, the authors include methods (e.g., cryopreservation) of optimizing sperm quality based on the etiology of azoospermia.
Assessment: This manual is an indispensable resource to all clinicians and researchers in male reproductive medicine. A novel publication of its kind, it focuses on optimizing the fertility potential of retrieved sperm utilized in the ART setting and brings a valuable, global review of the subject.

Doody's Review Service