Mass Spectrometry: Techniques for Structural Characterization of Glycans

by Michael A. Madson

Overview

Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules.

As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study.

• Authored by an analytical chemist with more than 30 years of experience in research and industry
• Serves as a quick reference in mass spectral analysis and carbohydrates
• Includes more than 60 figures to aid in the retention of key concepts

Product Details

ISBN-13:	9780128041352
Publisher:	Elsevier Science
Publication date:	05/06/2016
Sold by:	Barnes & Noble
Format:	eBook
Pages:	86
File size:	8 MB

About the Author

Mike Madson is CEO and Senior Research Scientist of BioLogistics, LLC, Iowa, USA

Table of Contents

• Introduction• Monosaccharide Composition Analysis• Elemental Analysis of MS Ions• Derivatization Techniques• Hydride Addition to Sulfate and phosphate- Chemistry• InstrumentationMALDI-TOF MSAPI 2000 MSICP ESI TOF MS• Hydride Insertion Addition Reaction for the Derivatization of Phosphorylated or Sulfated Glycans and the Discernment of Phosphate versus Sulfate Esters• Ester Substitution MS MethodThe Protocol for the Hydride Addition to the ethanol extract of banana fruitExample of MS2on Hydride added to the ethanol Extract of Banana Fruit Glycan• Glycan Isolation Methods• Other Glycan isolation Methods• Monosaccharide AnalysisAcid Hydrolysis• Mass Spectral Carbohydrate Structure Determination MethodMSMS/MS (MS2)MSnProposing Initial Structure Refining Methods for Determining Exact Mass StructureMS Instrumentation Requirements for Discovering Whole Molecule IonsDerivatization Method and Sulfate/Phosphate Discernment Method• References

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From the Publisher

This short-format reference presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrate molecules, thus providing the relevant research necessary for advances in this area of study