Table of Contents
[1]: Dynamic Green Fluorescent Protein Sensors for High-Content Analysis of the Cell Cycle
[2]: High-Content Fluorescence-Based Screening for Epigenetic Modulators
[3]: Development of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins
[4]: The Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan G Protein-Coupled Receptors by Arrestin Recruitment
[5]: High-Content Screening of Known G Protein-Coupled Receptors by Arrestin Translocation
[6]: Cell Imaging Assays for G Protein-Coupled Receptor Internalization: Application to High-Throughput Screening
[7]: High-Throughput Confocal Microscopy for ß-Arrestin–Green Fluorescent Protein Translocation G Protein-Coupled Receptor Assays Using the Evotec Opera
[8]: G Protein-Coupled Receptor Internalization Assays in the High-Content Screening Format
[9]: Screening for Activators of the Wingless Type/Frizzled Pathway by Automated Fluorescent Microscopy
[10]: A Live Cell, Image-Based Approach to Understanding the Enzymology and Pharmacology of 2-Bromopalmitate and Palmitoylation
[11]: High-Resolution, High-Throughput Microscopy Analyses of Nuclear Receptor and Coregulator Function
[12]: Tracking Individual Proteins in Living Cells Using Single Quantum Dot Imaging
[13]: Development and Application of Automatic High-Resolution Light Microscopy for Cell-Based Screens
[14]: Adenoviral Sensors for High-Content Cellular Analysis
[15]: Cell-Based Assays Using Primary Endothelial Cells to Study Multiple Steps in Inflammation
[16]: Development and Implementation of Multiplexed Cell-Based Imaging Assays
[17]: High-Throughput Screening for Modulators of Stem Cell Differentiation
[18]: High-Content Kinetic Calcium Imaging in Drug-Sensitive and Drug-Resistant Human Breast Cancer Cells
[19]: Measurement and Analysis of Calcium Signaling in Heterogeneous Cell Cultures
[20]: Multiplex Analysis of Inflammatory Signaling Pathways Using a High-Content Imaging System
[21]: Generation and Characterization of a Stable MK2-EGFP Cell Line and Subsequent Development of a High-Content Imaging Assay on the Cellomics ArrayScan Platform to Screen for p38 Mitogen-Activated Protein Kinase Inhibitors
[22]: Development and Implementation of Three Mitogen-Activated Protein Kinase (MAPK) Signaling Pathway Imaging Assays to Provide MAPK Module Selectivity Profiling for Kinase Inhibitors: MK2-EGFP Translocation, c-Jun, and ERK Activation
[23]: Assay Development and Case History of a 32K-Biased Library High-Content MK2-EGFP Translocation Screen to Identify p38 Mitogen-Activated Protein Kinase Inhibitors on the ArrayScan 3.1 Imaging Platform
[24]: Compound Classification Using Image-Based Cellular Phenotypes
[25]: High-Content Screening: Emerging Hardware and Software Technologies
[26]: An Infrastructure for High-Throughput Microscopy: Instrumentation, Informatics, and Integration
[27]: Protein Translocation Assays: Key Tools for Accessing New Biological Information with High-Throughput Microscopy
[28]: High-Content Screening of Functional Genomic Libraries
[29]: Fluorescent Protein-Based Cellular Assays Analyzed by Laser-Scanning Microplate Cytometry in 1536-Well Plate Format
[30]: High-Throughput Measurements of Biochemical Responses Using the Plate::Vision Multimode 96 Minilens Array Reader
[31]: Systems Cell Biology Based on High-Content Screening
[32]: Digital Autofocus Methods for Automated Microscopy
[33]: Fluorescence Lifetime Imaging Microscopy: Two-Dimensional Distribution Measurement of Fluorescence Lifetime
Author Index
Subject Index