Metastasis Research Protocols / Edition 1

by Susan A. Brooks, Udo Schumacher

Overview

The process of metastasis formation is hugely complex, as described in the introductory chapter of this book, and this complexity has led us to compile two volumes of methods, from a vastly divergent background that attempts to encompass the whole spectrum of cancer biology. This first volume, Metastasis Research Prools: Analysis of Cells and Tissues, concentrates on analysis and mapping of molecules produced by cells and tissues and analysis of the molecular biology underlying their expression, whereas the second volume, Metastasis Research Prools: Cell Behavior In Vitro and In Vivo,focuses sharply on the determination of cell behavior in vitro and in vivo. We have deliberately included chapters describing well-established and familiar te- niques (for example, SDS-PAGE and Western blotting [Chapter 11], and immunocyhemistry [Chapter 2]) in addition to the newer and more speci- ized approaches and specific examples of their application, because—although the methodology is readily available in the published literature and established in many laboratories—we wished these volumes to “stand alone” and to make accessible here the standard techniques that underpin much metastasis research for both the newcomer to the field and the seasoned researcher. Undoubtedly, owing to the complexity of the metastatic cascade and the wealth of research techniques involved in scientific approaches to its unraveling, and despite our best efforts to make these volumes as comprehensive as seems feasible, this is a tall order, and there will inevitably be omissions. For these we apologize.

Product Details

ISBN-13:	9781617370960
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/09/2010
Series:	Methods in Molecular Medicine , #57
Edition description:	Softcover reprint of hardcover 1st ed. 2001
Pages:	344
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

About the Author

Brooks, Susan A.; Schumacher, Udo

Table of Contents

Detection Methods for Cellular Markers of Metastatic Potential.- Histopathological Assessment of Metastasis.- Basic Immunocyhemistry for Light Microscopy.- Multiple Labeling Techniques for Fluorescence Microscopy.- Lectin Hishemistry to Detect Altered Glycosylation in Cells and Tissues.- Immunocyhemical Detection and Characterization of Individual Micrometastatic Tumor Cells.- Understanding Metastasis Through Integrin Expression.- Surrogate Markers of Angiogenesis and Metastasis.- Measurement of Microvessel Density in Primary Tumors.- Assessment of Cellular Proliferation by Calculation of Mitotic Index and by Immunohishemistry.- Flow Cytometric Measurement of Cell Proliferation.- SDS -PAGE and Western Blotting Techniques.- Assessment of Gelatinases (MMP-2 and MMP-9 by Gelatin Zymography.- Genetic Aspects of Metastasis.- In Situ Hybridization to Localize mRNAs.- Fluorescence itIn Situ Hybridization.- Comparative Genomic Hybridization.- RNA Purification and Quantification Methods.- Northern and Southern Blotting and the Polymerase Chain Reaction to Detect Gene Expression.- Methylation Analysis of CpG Islands.- Detection of MetastaticTumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction.- Differential Display.- Mathematical Modeling of Metastasis.- Predictive Mathematical Modeling in Metastasis.

Editorial Reviews

4 Stars! from Doody

This book is a valuable addition to the literature as it brings together key people in the field of cancer metastasis to provide practical step-by-step prools. . . This book is written in such as way that it would be useful to beginning students and to anyone curious about the methods that are available to study the key steps in cancer metastasis. This book would also be useful to seasoned researchers as it brings together a number of different methodologies in one easy-to-read book. . . This is a very practical book covering areas of research in cancer invasion and metastasis. The greatest strength of the book is in collecting the different prools that are useful in studying cellular behavior in one easy-to-read book.."-Weighted Numerical Score: 91 - 4 Stars!-Doody's Health Sciences Book Review Journal

"These volumes should be of interest for all medical and academic libraries where cancer research is performed." - E-Streams

From the Publisher