Microbial Biofilms: Methods and Protocols

by Gianfranco Donelli

Overview

The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Prools provides a detailed description of the currently available methods and prools to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory prools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Microbial Biofilms: Methods and Prools presents readers with the most established and validated experimental procedures to investigate microbial biofilms.

Product Details

ISBN-13:	9781493946761
Publisher:	Springer New York
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #1147
Edition description:	Softcover reprint of the original 1st ed. 2014
Pages:	380
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Methods for Dynamic Investigations of Surface Attached in vitro Bacterial and Fungal Biofilms.- Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms.- Quorum Sensing in Gram-Positive Bacteria: Assay Prools for Staphylococcal agr and enterococcal fsr Systems.- Advanced Techniques for in situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy.- Multiplex Fluorescence in situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyse Multispecies Oral Biofilms.- Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial Infections.- Experimental Approaches to Investigating the Vaginal Biofilm Microbiome.- Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopaedic Infections.- Animal Models to Evaluate Bacterial Biofilm Development.- Animal Models to Investigate Fungal Biofilm Formation.- Non-Mammalian Model Systems to Investigate Fungal Biofilms.- Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors.- In vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development.- Biofilm Matrix-Degrading Enzymes.- Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices.- Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection Therapy.- Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa in vitro and in vivo.- Contribution of Confocal Laser Scanning Microscopy in Deciphering Biofilm Tridimensional Structure and Reactivity.- Chip-Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibiotics and Biological Agents.- Bacteriophage Attack as an Anti-Biofilm Strategy.- Photodynamic Therapy as a Novel Antimicrobial Strategy Against Biofilm-Based Nosocomial Infections: Study Prools.- Capturing Air-Water Interface Biofilms for Microscopy and Molecular Analysis.- Biofilm-Growing BacteriaInvolved in the Corrosion of Concrete Wastewater Pipes: Prools for Comparative Metagenomic Analyses.- Culture-Independent Methods to Study Subaerial Biofilm Growing on Biodeteriorated Surfaces of Cultural Heritage Stone Materials and Frescoes.- Biofilms of Thermophilic Bacilli Isolated from Dairy Processing Plants and Efficacy of Sanitizers.

Editorial Reviews

From the book reviews:

“This book … is organized in three parts: Investigations on biofilms in health and disease, Investigations on anti-biofilm compounds and strategies, and Investigations on biofilms in the environment and manufacturing plants. … this book should be read by students, researchers, and teachers working in the field of biofilms, as well as scientists working in Microbiology, Ecology or Medicine.” (Ioan I. Ardelean, Bulletin of Micro and Nanoelectrotechnologies, Vol. 5 (3-4), December, 2014)

From the Publisher