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Microbiology: A Laboratory Manual / Edition 11
- ISBN-10:
- 0134098633
- ISBN-13:
- 9780134098630
- Pub. Date:
- 01/22/2016
- Publisher:
- Pearson
![Microbiology: A Laboratory Manual / Edition 11](http://img.images-bn.com/static/redesign/srcs/images/grey-box.png?v11.9.4)
Microbiology: A Laboratory Manual / Edition 11
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Overview
For courses in Microbiology Lab and Nursing and Allied Health Microbiology Lab
A Flexible Approach to the Modern Microbiology Lab
Easy to adapt for almost any microbiology lab course, this versatile, comprehensive, and clearly written manual is competitively priced and can be paired with any undergraduate microbiology text. Known for its thorough coverage, straightforward procedures, and minimal equipment requirements, the Eleventh Edition incorporates current safety protocols from governing bodies such as the EPA, ASM, and AOAC. The new edition also includes alternate organisms for experiments for easy customization in Biosafety Level 1 and 2 labs. New lab exercises have been added on Food Safety and revised experiments, and include options for alternate media, making the experiments affordable and accessible to all lab programs. Ample introductory material, engaging clinical applications, and laboratory safety instructions are provided for each experiment along with easy-to-follow procedures and flexible lab reports with review and critical thinking questions.Product Details
ISBN-13: | 9780134098630 |
---|---|
Publisher: | Pearson |
Publication date: | 01/22/2016 |
Edition description: | Lab Manual |
Pages: | 560 |
Product dimensions: | 9.00(w) x 10.80(h) x 0.70(d) |
About the Author
James G. Cappuccino is a retired professor emeritus of microbiology from the Department of Biology of the State University of New York at Rockland Community College in Suffern, New York. He received his B.S degree from Seton Hall University in 1951, his M.S degree (1955) and his Ph.D. (1957) in microbiology from Rutgers University in New Brunswick, New Jersey. He was the author and co-author of numerous papers in the area of cancer research, and was a member of the faculty of the Sloan Kettering Division of the Graduate school of Medical Sciences at Cornell University where he taught microbiology from 1957-1970. From there, he taught microbiology, parasitology and clinical chemistry at SUNY Rockland until 2008. He was awarded the status of emeritus professor in 2012. In 1991 he was the recipient of the Chancellor’s award from the State University of New York for Excellence in Teaching. He is an emeritus member of the American Society for Cancer Research (ASCR) and an emeritus member of American society for Microbiology (ASM). When not writing he enjoys spending time with his wife Elaine and their family at their summer home at the New Jersey shore. He also enjoys theater, literature, and the quiet hour in his wood working shop.
Chad T. Welsh holds a Ph.D. in Microbiology and Immunology from the University of Louisville, School of Medicine, also an M.S. and B.S. in Biology from Middle Tennessee State University. Currently he is the Chair of the Division of Biological and Earth Sciences at Lindenwood University in St. Charles, MO where he has the privilege of teaching Microbiology, both for non-majors and majors, Cellular Immunology, Parasitology, and many other courses since 2010. His research interests fall within bacteriology, eukaryotic cell biology, and immunology, focusing primarily on intracellular eukaryotic signals in response to pulmonary bacterial pathogens. His mentored research projects with his students have spanned the interest areas of soil microbial ecology, immune stress responses in collegiate athletes, oral bacterial flora communities, and many others.
Table of Contents
Laboratory Safety
Laboratory Protocol
Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms
1. Culture Transfer Techniques
2. Techniques for Isolation of Pure Cultures
3. Cultural Characteristics of Microorganisms
Part 2 Microscopy
4. Microscopic Examination of Stained Cell Preparations
5. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount
Part 3 Bacterial Staining
6. Preparation of Bacterial Smears
7. Simple Staining
8. Negative Staining
9. Gram Stain
10. Acid-Fast Stain
11. Differential Staining for Visualization of Bacterial Cell Structures
Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations
12. Nutritional Requirements: Media for the Routine Cultivation of Bacteria
13. Use of Differential, Selective, and Enriched Media
14. Physical Factors: Temperature
15. Physical Factors: pH of the Extracellular Environment
16. Physical Factors: Atmospheric Oxygen Requirements
17. Techniques for the Cultivation of Anaerobic Microorganisms
18. Serial Dilution—Agar Plate Procedure to Quantitate Viable Cells
19. The Bacterial Growth Curve
Part 5 Biochemical Activities of Microorganisms
20. Extracellular Enzymatic Activities of Microorganisms
21. Carbohydrate Fermentation
22. Triple Sugar—Iron Agar Test
23. IMViC Test
24. Hydrogen Sulfide Test
25. Urease Test
26. Litmus-Milk Reactions
27. Nitrate Reduction Test
28. Catalase Test
29. Oxidase Test
30. Utilization of Amino Acids
31. Genus Identification of Unknown Bacterial Cultures
Part 6 The Protozoa
32. Free-Living Protozoa
33. Parasitic Protozoa
Part 7 The Fungi
34. Cultivation and Morphology of Molds
35. Yeast Morphology, Cultural Characteristics, and Reproduction
36. Identification of Unknown Fungi
Part 8 The Viruses
37. Cultivation and Enumeration of Bacteriophages
38. Isolation of Coliphages from Raw Sewage
39. Propagation of Isolated Bacteriophage Cultures
Part 9 Physical and Chemical Agents for the Control of Microbial Growth
40. Physical Agents of Control: Moist Heat
41. Physical Agents of Control: Electromagnetic Radiations
42. Chemical Agents of Control: Chemotherapeutic Agents
43. Determination of Penicillin Activity in the Presence and Absence of Penicillinase
44. Chemical Agents of Control: Disinfectants and Antiseptics
Part 10 Microbiology of Food
45. Microbiological Analysis of Food Products: Bacterial Count
46. Microbial Fermentation
Part 11 Microbiology of Water
47. Standard Qualitative Analysis of Water
48. Quantitative Analysis of Water: Membrane Filter Method
Part 12 Microbiology of Soil
49. Microbial Populations in Soil: Enumeration
50. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
51. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique
Part 13 Bacterial Genetics
52. Enzyme Induction
53. Bacterial Conjugation
54. Isolation of a Streptomycin-Resistant Mutant
55. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
Part 14 Biotechnology
56. Bacterial Transformation
57. Isolation of Bacterial Plasmids
58. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA
Part 15 Medical Microbiology
59. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries
60. Normal Microbial Flora of the Throat and Skin
61. Identification of Human Staphylococcal Pathogens
62. Identification of Human Streptococcal Pathogens
63. Identification of Streptococcus pneumoniae
64. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems
65. Isolation and Presumptive Identification of Campylobacter
66. Microbiological Analysis of Urine Specimens
67. Microbiological Analysis of Blood Specimens
68. Species Identification of Unknown Bacterial Cultures
Part 16 Immunology
69. Precipitin Reaction: The Ring Test
70. Agglutination Reaction: The Febrile Antibody Test
71. Enzyme-Linked Immunosorbent Assay
72. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures
Appendix 1. Scientific Notation
Appendix 2. Methods for the Preparation of Dilutions
Appendix 3. Microbiological Media
Appendix 4. Biochemical Test Reagents
Appendix 5. Staining Reagents
Appendix 6. Experimental Microorganisms