MicroRNA Profiling: Methods and Protocols

by Sweta Rani (Editor)

Overview

This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

Product Details

ISBN-13:	9781071628256
Publisher:	Springer US
Publication date:	11/29/2023
Series:	Methods in Molecular Biology , #2595
Edition description:	2nd ed. 2023
Pages:	252
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

MiRNA Biogenesis and Regulation of Diseases: An Overview.- Assessment of Basic Biological Functions Exerted by miRNAs.- Extraction of miRNAs from Formalin Fixed Paraffin Embedded (FFPE) Tissues.- MiRNA Isolation from Plants Rich in Polysaccharides and Polyphenols.- MicroRNA Profiling of Exosomes.- MiRNA Profiling in Human Induced Pluripotent Stem Cells.- MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells.- TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery.- Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.- Exosomal MicroRNA Discovery in Age-Related Macular Degeneration.- Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts.- Circulating MicroRNAs in Cancer.- Profiling Circulating MiRNAs from the Plasma of Individuals with Metabolic Syndrome.- Manipulating MiRNA Expression to Uncover Hidden Functions.- Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow Fractionation.- MicroRNA Expression Profiling Using Agilent One-Color Microarray.- A Multiplex Ligation Assay for MiRNA Copy Number Profiling.- Practical Bioinformatics Analysis of MiRNA Data Using Online Tools.- Visualization and Analysis of MiRNA-Targets Interactions Networks.- Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.- Customization of Artificial MicroRNA Design.