Molecular Histochemical Techniques / Edition 1

by Takehiko Koji

Overview

Molecular Hishemical Techniques presents detailed prools for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. In situ nick translation and TUNEL are described in detail, along with radioactive and nonradioactive methods for in situ hybridization. Of special significance is the description of Southwestern hishemistry, a new development in molecular hishemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular hishemistry along with practical how-to-do-it details. Methodology is set forth step-by-step in easy-to-follow language by leading scientists who are working in the field today. The book serves as both a rich source of the latest information and a practical lab manual.

Product Details

ISBN-13:	9784431702313
Publisher:	Springer Japan
Publication date:	05/25/2000
Series:	Springer Lab Manuals
Edition description:	2000
Pages:	263
Product dimensions:	6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

1 Introduction to the Detection of DNA Strand Breaks.- 2 In Situ Nick Translation.- 3 In Situ Nick End-Labeling: Light Microscopical.- 4 In Situ Nick End-Labeling: Electron Microscopical.- 5 Introduction to the Detection of Specific DNA and RNA Sequences.- 6 In Situ Hybridization for DNA: Fluorescent Probe.- 7 In Situ Hybridization for RNA: Radioactive DNA Probe.- 8 In Situ Hybridization for RNA: Radioactive RNA Probe — A Prool for Practice With a Kit.- 9 In Situ Hybridization for RNA: Nonradioactive probe: ds cDNA Probe.- 10 In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe.- 11 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: T-T Dimer.- 12 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (I).- 13 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (II).- 14 In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe.- 15 Electron Microscopic in Situ Hybridization and its Combination With Immunohishemistry.- 16 Whole Mount in Situ Hybridization for mRNA detection in Chick Embryos.- 17 Introduction to the Detection of Transcription Regulatory Proteins.- 18 Southwestern Hishemistry.