Molecular Methods for Virus Detection

by Danny L. Wiedbrauk (Editor), Daniel H. Farkas (Editor)

Overview

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.

Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies.

• Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions
• Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT
• Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence
• Includes quality control information crucial in research and clinical laboratories
• Most chapters are written by the inventors and principal developers of the methodologies
• Includes color plates, 77 figures, and 18 tables

Product Details

ISBN-13:	9780080536897
Publisher:	Elsevier Science
Publication date:	02/08/1995
Sold by:	Barnes & Noble
Format:	eBook
Pages:	386
File size:	8 MB

About the Author

Wiedbrauk, Danny L. (William Beaumont Hospital, Royal Oak, MI); Farkas, Daniel H. (William Beaumont Hospital, Royal Oak, MI)

The contributors represent the specialties of biomedical research, pathology, pediatrics, and molecular and cellular biology. Most are from research institutions and medical laboratories in the U.S. Institutions prominently represented include Becton Dickinson Research Center in North Carolina, Immune Response Corporation in California, and Walter Reed Army Institute of Research.

Table of Contents

D.L. Wiedbrauk and A.M. Drevon, Nucleic Acid Detection Methods.
D.L. Wiedbrauk and J. Stoerker, Quality Assurance in the Molecular Biology Laboratory.
D.L. Stoler and N.L. Michael, Nucleic Acid Blotting Techniques for Virus Detection.
J.Carr, In Situ Hybridization.
R.L. Hodinka, Antiviral Susceptibility Testing Using DNA-DNA Hybridization.
J.C. Wilbur and M.S. Urdea, Quantitation of Viral Nucleic Acids Using Branched DNA (bDNA) Signal Amplification.
I. Bronstein and C.E.M. Olesen, Chemiluminescence Methods.
B.J. McCreedy, Polymerase Chain Reaction.
F. Ferre, P. Pezzoli, E. Buxton, C. Duffy, A. Marchese, and A. Diagle, Quantitation of RNA Targets Using the Polymerase Chain Reaction.
J.B. Mahony and M.A. Chernesky, Multiplex Polymerase Chain Reaction (M-PCR).
G.J. Nuovo, PCR in Situ Hybridization.
R. Sooknanan, B. van Gemen, and L.T. Malek, Nucleic Acid Sequence Based Amplification (NASBA(tm)).
S.S.Ghosh, E. Fahy, and T.R. Gingeras, The Self-Sustained Sequence Replication (3SR) Reaction and its Application in Clinical Diagnostics and Molecular Biology.
J.D. Burczak, S. Ching, H.-Y. Hu, and H.H. Lee, Ligase Chain Reaction (LCR) for the Detection of Infectious Agents.
G. T. Walker, et al., A Chemiluminescent DNA Probe Test Based upon Strand Displacement Amplification (SDA).
D.M. Schuster, M.S. Berninger, and A. Rashtchian, Ligation Activated Transcription Amplification: Amplification and Detection of Human Papillomaviruses.
Subject Index.

Editorial Reviews

3 Stars from Doody

In this new book, the authors have assembled descriptions and protocols of some of the most promising innovative molecular techniques that have potential application to diagnostic virology. "The purpose is to showcase these new approaches to solve old problems as well as to summarize and update some of the basics of nucleic acid isolation, amplification, and detection. The book is easy to follow and is well indexed so that specific information can be found quickly. "Virologists from academia to industry will find the book interesting and informative. This is not a basic reference book. "The protocol designs are well presented but, in some cases, intimate details of basic procedures are, by necessity, not discussed. A key issue is that the authors address the limits of sensitivity and the advantages and disadvantages of the various techniques. It is highly informative to get the perspective of each of the contributors. The need for appropriate controls and laboratory procedures is emphasized for each technique, and examples are provided. "The book contains a compilation of very useful information if you know what to look for. It is difficult to know how many of these applications will be routinely used in the diagnostic laboratory, but the book clearly points to the way of the future.

William J. O'Brien

A handbook that assembles a wide variety of methods that have been, or soon will be, employed in the clinical virology laboratory for using molecular diagnostic procedures to detect viral infections. Chapter 1 provides an introduction to these molecular diagnostic methods, while Chapter 2 describes the important elements of a quality assurance program for the molecular virology laboratory. A unique feature is the presence of clinical detection protocols for seven emerging diagnostic methodologies, written by the inventors or principal developers of the technologies. Wire comb-binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

Reviewer: William J. O'Brien, PhD (Medical College of Wisconsin)
Description: In this new book, the authors have assembled descriptions and protocols of some of the most promising innovative molecular techniques that have potential application to diagnostic virology.
Purpose: The purpose is to showcase these new approaches to solve old problems as well as to summarize and update some of the basics of nucleic acid isolation, amplification, and detection. The book is easy to follow and is well indexed so that specific information can be found quickly.
Audience: Virologists from academia to industry will find the book interesting and informative. This is not a basic reference book.
Features: The protocol designs are well presented but, in some cases, intimate details of basic procedures are, by necessity, not discussed. A key issue is that the authors address the limits of sensitivity and the advantages and disadvantages of the various techniques. It is highly informative to get the perspective of each of the contributors. The need for appropriate controls and laboratory procedures is emphasized for each technique, and examples are provided.
Assessment: The book contains a compilation of very useful information if you know what to look for. It is difficult to know how many of these applications will be routinely used in the diagnostic laboratory, but the book clearly points to the way of the future.

Doody's Review Service

"A particular strength of many of the chapters is the emphasis which has been placed on identifying problems that can arise when introducing and performing the tests and discussion of relative advantages and disadvantages... The clearly presented protocols should enable many research and development departments to develop in house assays." —D. Cubitt, in THE JOURNAL OF CLINICAL PATHOLOGY

From the Publisher