Mouse Genetics: Concepts and Applications / Edition 1

by Lee M. Silver

Overview

Mouse Genetics offers for the first time in a single comprehensive volume a practical guide to mouse breeding and genetics. Nearly all human genes are present in the mouse genome, making it an ideal organism for genetic analyses of both normal and abnormal aspects of human biology. Written as a convenient reference, this book provides a complete description of the laboratory mouse, the tools used in analysis, and procedures for carrying out genetic studies, along with background material and statistical information for use in ongoing data analysis. It thus serves two purposes, first to provide students with an introduction to the mouse as a model system for genetic analysis, and to give practicing scientists a detailed guide for performing breeding studies and interpreting experimental results. All topics are developed completely, with full explanations of critical concepts in genetics and molecular biology. As investigators around the world are rediscovering both the heuristic and practical value of the mouse genome, the demand for a succinct introduction to the subject has never been greater. Mouse Genetics is intended to meet the needs of this wide audience.

Product Details

ISBN-13:	9780195075540
Publisher:	Oxford University Press
Publication date:	04/13/1995
Pages:	376
Product dimensions:	6.42(w) x 9.41(h) x 1.12(d)

About the Author

Princeton University

Table of Contents

1. An Introduction to Mice1.1. Of Mice, Men, and a Woman1.2. The Origin of Mice in Genetic Research1.3. The New Biology and the Mouse Model2. Town Mouse, Country Mouse2.1. What are Mice? 2.2. Where Do Mice Come From? 2.3. The Mus Species Group and the House Mouse2.4. Lifestyles and Adaptability of Wild House Mice3. Laboratory Mice3.1. Sources of Laboratory Mice3.2. Mouse Crosses and Standard Strains3.3. Coisogenics, Congenics, and Other Specialized Strains3.4. Standardized Nomenclature3.5. Strategies for Record-Keeping4. Reproduction and Breeding4.1. Reproductive Performance: Comparison of Inbred Strains4.2. Germ Cell Differentiation and Sexual Maturation4.3. Mating and Pregnancy4.4. The Postnatal Period4.5. Assisted Reproduction for the Infertile Cross5. The Mouse Genome5.1. Quantifying the Genome5.2. Chromosomes5.3. Genome Evolution and Gene Families5.4. Repetitive "Non-Functional" DNA Families5.5. Genomic Imprinting6. Mutagenesis and Transgenesis6.1. Classical Mutagenesis6.2. Embryo Manipulation6.3. Transgenic Mice Formed by Nuclear Injection6.4. Targeted Mutagenesis and Gene Replacement6.5. Further Uses of Transgenic Technologies7. Mapping in the Mouse: An Overview7.1. Genetic Maps Come in Various Forms7.2. Mendel's Genetics, Linkage, and the Mouse7.3. General Strategies for Mapping Mouse Loci7.4. The Final Chapter of Genetics8. Genetic Markers8.1. Genotypic and Phenotypic Variation8.2. Restriction Fragment Length Polymorphisms (RFLPs)8.3. Polymorphisms Detected by PCR8.4. Region-Specific Panels of DNA Markers9. Classical Linkage Analysis and Mapping Panels9.1. Demonstration of Linkage in the Mouse9.2. Recombinant Inbred Strains9.3. Interspecific Mapping Panels9.4. Starting From Scratch With a New Mapping Project9.5. Quantitative Traits and Polygenic Analysis10. Non-Breeding Mapping Strategies10.1. Linkage Maps Without Breeding10.2. Chromosomal Mapping Tools10.3. Physical Maps and Positional Cloning10.4. The Human Genome Project and the Ultimate MapAppendix A: Suppliers of Mice. Appendix B: Computational Tools and Electronic Databases. Appendix C: Source Materials for Further Reading. Appendix D: Statistics. Appendix E: Glossary of Terms.

Editorial Reviews

"Mapping the human genome and identifying genes that contribute to susceptibility and resistance to disease are currently major preoccupations in biomedical science. The foundations for much of this sophisticated field of research were established in the mouse . . . . Lee Silver in a pleasing and easy style presents a unique synthesis of modern molecular genetics and the biology of the laboratory mouse . . . . Provides exciting reading for the student and continuing education for the more applied worker . . . . An exciting introduction to the comprehensive biology that is involved in the intriguing mysteries of the evolutionary process." —Science

"Students and researchers involved in mouse genetic research will find Silver's book a welcome addition to their shelves." —Choice

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