Mycotoxin Protocols / Edition 1

by Mary W. Trucksess, Albert E. Pohland

Overview

Mycotoxins produced by molds are common contaminants of many important crops, including wheat, corn, rice, and peanuts. Some mycotoxins are found in fruits and vegetables. These contaminants have a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. The occurrence of mycotoxins in foods is an unavoidable worldwide problem. About 80 countries have imposed regulatory limits to minimize human and animal exposure to mycotoxins. Regulatory limits, including international standards, have tremendous economic impact and must be developed using science-based risk assessments. The purpose of Mycotoxin Prools is to provide the scientific and technological basis for analytical methods for use in obtaining the exposure data needed for risk assessments. Mycotoxin Prools is divided into four sections, which are interc- nected. The first section: Chapters 1–5 describe the general techniques for mycotoxin analysis with emphasis on the importance of method validation based on statistical parameters; sampling procedures for collecting a sample as representative as possible of a bulk lot; the isolation of mycotoxins for use as analytical standards or for toxicological studies; the evaluation of purity and preparation of standards; and the detection and identification of impu- ties in isolated mycotoxins. Sections 2–4: Chapters 6–19 describe the most current chromatographic and immunochemical methods for studies on the major mycotoxins.

Product Details

ISBN-13:	9780896036239
Publisher:	Springer-Verlag New York, LLC
Publication date:	09/06/2000
Series:	Methods in Molecular Biology , #157
Edition description:	2001
Pages:	244
Product dimensions:	6.10(w) x 9.25(h) x 0.02(d)

About the Author

Trucksess, Mary W. (Food and Drug Administration); Pohland, Albert E. (Food and Drug Administration)

Table of Contents

General Techniques.- Mycotoxin Method Evaluation.- Sampling Techniques.- 3 Preparatory Isolation of Mycotoxins from Solid Phase Fungal Cultures.- 4 Preparation of Mycotoxin Standards.- Electrospray Mass Spectrometry for Mycotoxin Detection and Purity Analysis.- Methods for Aspergillus Toxins.- Measurement of Aflatoxins Using Capillary Electrophoresis.- Liquid Chromatographic Method for Aflatoxin M1 in Milk.- Immunochemical Method for Cyclopiazonic Acid.- Immunochemical Method for Ochratoxin A.- Methods for Fusarium Toxins.- Solution Fluorometric Method for Deoxynivalenol in Grains.- Chromatographic Methods forTrichothecenes.- Chromatographic Method for the Determination of the Mycotoxin Moniliformin in Corn.- Liquid Chromatographic Method for Fumonisins in Corn.- Enzyme-Linked Immunosorbent Assays of Zearalenone Using Polyclonal, Monoclonal and Recombinant Antibodies.- Methods for Other Fungal Toxins.- 15 Chromatographic Method for StachybotrysToxins.- 16 Immunochemical Method for Citrinin.- Solid Phase Extraction Method for Patulin in Apple Juice and Unfiltered Apple Juice.- Liquid Chromatographic Method for the Determination of Ergot Alkaloids in Cereal Grains.- Chromatographic Method for AlternariaToxins in Apple Juice.

Editorial Reviews

Experienced scientists detail their methods for determining the levels of mycotoxin contamination in human food and animal feed, nearly half of them involving molecular-based immunochemical or immunochemical-chromatographic techniques. They describe the necessary equipment, reagents, and procedures for analyzing a wide range of specific toxins, and explain general techniques, sampling procedures for collecting representative test samples, isolating, and detecting and measuring. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

"...many analysts will be attracted by this book in which assays for several mycotoxins are described." - MicroBiology Today

"After reading the complete book, I would like to congratulate the editors as well as all the authors for work well-done. This book is well-written and could be used both as a practical laboratory handbook as well as a reference book for research scientists. The authors have not only described the prools in detail, but also reviewd different methodologies and provided notes of their own experience in some of the prools. These notes are extremely useful for the readeres to understand the rationales, difficulties, steps to overcome the problems, alternatives for certain steps, precautions for certain steps as well as approaches to modify some steps/prools for other related mycotoxins. " - Journal of Food Biochemistry

From the Publisher