Neural Cell Culture: A Practical Approach / Edition 1

by James Cohen, Graham P. Wilkin

Overview

The immense complexity and cellular heterogeneity of the vertebrate nervous system makes it difficult to study many types of neural processes at a cellular and molecular level. In recent years, the establishment of cell culture model systems for almost every region and cell type in the nervous system has been crucial in facilitating such studies and in heralding some key breakthroughs in our understanding of the underlying mechanisms.
Neural Cell Culture brings together methods for preparing cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems of mammalian and avian species. Important features of this volume are the clearly illustrated dissection guides accompanying each chapter, methods for enriching and purifying distinct cell types, and guidance on optimizing cell viability and growth. Each chapter includes valuable step-by-step protocols, hints and tips for success, advice, and background information.

Product Details

ISBN-13:	9780199634842
Publisher:	Oxford University Press
Publication date:	05/02/1996
Series:	Practical Approach Series , #165
Pages:	268
Product dimensions:	6.13(w) x 9.25(h) x 0.59(d)

About the Author

Cohen, James (Guy's Hospital, London); Wilkin, Graham P. (Imperial Coll of Science, Technology and Medicine, London)

The contributors represent the specialties of neurology, neurobiology, biology, medicine, anatomy, and biomedical sciences. Most are from academic medical centers in the U.S., the U.K., and France. Institutions prominently represented include Imperial Coll of Science, Technology, and Medicine in London, Univ of Iowa, Universite Paris, Univ Coll London, and Institute of Neurology.

Table of Contents

I. CNS Neurons1. Cerebellum: granule cells2. Cerebellar Purkinje neurons3. Nigral and Striated neurons4. Septal neurons5. Proliferation & differentiation in retinal cell cultures6. Purified embryonic motoneuronsII. PNS glia and non-neuronal cells7. Astrocytes8. Oligodendrocyte lineage cells from neonatal rat brain9. Microglia: the tissue macrophage of the CNS10. Brain endotheliumIII. PNS neurons and glia11. Neural crest cells: strategies to generate lineage diversification in vitro13. Avian cilliary and lumbar sympathetic ganglion neurons14. Enteric ganglion cells15. Mouse olfactory epithelium16. Schwann cell culture