Neural Stem Cells: Methods and Protocols / Edition 2

by Leslie P. Weiner

Overview

Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory prools will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

Product Details

ISBN-13:	9781588298461
Publisher:	Springer-Verlag New York, LLC
Publication date:	02/01/2008
Series:	Methods in Molecular Biology , #438
Edition description:	2nd ed. 2008
Pages:	406
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Definitions and Criteria for Stem Cells.- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard.- Isolation and Culture of NSCs.- Neural Differentiation of Human Embryonic Stem Cells.- Generating Neurons from Stem Cells.- Isolation of Neural Stem and Precursor Cells from Rodent Tissue.- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization.- Adult Neural Stem Cells.- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb.- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium.- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property.- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood.- Production of Neurospheres from CNS Tissue.- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells.- In Vitro Studies: A. Cellular Techniques.- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures.- Telomere Neurobiology.- Chromosomal Mosaicism in Neural Stem Cells.- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype.- In Vitro Migration Assays of Neural Stem Cells.- Measuring Apoptosis in Neural Stem Cells.- Markers of Adult Neural Stem Cells.- In Vitro Studies: B. Molecular Techniques.- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR.- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells.- Electroporation Strategies for Genetic Manipulation and Cell Labeling.- Novel and Immortalization-Based Prools for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches.- Studies for Transplantation.- Identification of Newborn Cells by BrdU Labeling and Immunocyhemistry In Vivo.- Immunocyhemical Analysis of Neuronal Differentiation.- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold.- Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS.- Optimizing Stem Cell Grafting into the CNS.- Routes of Stem Cell Administration in the Adult Rodent.

Editorial Reviews

Reviewer: Celso Agner, MD, MS, MSc (Michigan Neurology Partners)
Description: Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. In addition, potential cure of degenerative neurological disorders has challenged both scientists and the lay community. Since the first edition of this book, much has changed in the laboratory techniques and understanding of neural stem cells. A second edition was, therefore, much needed.
Purpose: The purpose is to clarify research protocols in the investigation of neural stem cells. These worthy objectives are met.
Audience: Neurologists, neurosurgeons, and neuroscientists are the main audience.
Features: Divided into four sections, the book addresses cell culture, cellular, molecular, and transplantation techniques. Despite being primarily a technical book outlining many bench laboratory techniques, the last section describes a possible use for stem cells in the management of neurological conditions in the future. Neural stem cell transplantation may find its way to control neoplasms, aggressive multiple sclerosis, Parkinson's disease, and other conditions in the decades to come.
Assessment: This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries.

Doody's Review Service

From the reviews of the second edition:

"Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. … The purpose is to clarify research prools in the investigation of neural stem cells. These worthy objectives are met. Neurologists, neurosurgeons, and neurosciences are the main audience. … This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries." (Celso Agner, Doody’s Review Service, June, 2008)

"Neural Stem Cells: Methods and Prools, Second Edition, provides a comprehensive overview on cutting-edge methods for scientists working on questions related to neural stem-cell biology. … The volume outlines methods and prools that allow investigating mechanisms, which regulate self-renewal, cell survival and differentiation of NSCs. A better understanding of these processes is of great importance for future successful applications of NSCs in neurodegenerative diseases and injuries." (S. Atanasoski, Schweizer Archiv für Neurologie und Psychiatrie, April, 2009)

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