Table of Contents
Introductory remarks at the overview session.- Nitrogenases: Distribution, composition, structure and function.- Rhizobium and legume nodulation: A molecular dialogue.- Plant function in nodulation and nitrogen fixation in legumes.- Organization and regulation of nitrogen fixation genes.- The role of respiration in symbiotic nitrogen fixation.- Evolution since Knoxville: Were nitrogen-fixing organisms wise to inhabit land plants?.- An integrated, interdisciplinary approach to understanding the chemistry of nitrogenases.- Structures and functions of the nitrogenase proteins.- The crystal structure of the nitrogenase MoFe protein from Clostridium pasteurianum.- Kinetics and mechanism of nitrogenase — a role for ‘P’ centres in dinitrogen reduction.- Nif gene products and their roles in nitrogen fixation.- Intermolecular electron transfer and substrate reduction properties of MoFe proteins altered by site-specific amino acid substitution.- Use of Fe protein altered at specific amino acid positions to probe its function in nitrogenase catalysis.- Metal regulation of nitrogenases and molybdenum transport in Azotobactervinelandii.- Regulatory role of molecular chaperones in nitrogenase biogenesis.- Nod factors from Rhizobium fredii USDA257.- The sulfate group on the reducing end protects nod signals of R. meliloti against hydrolysis by Medicaqo chitinases.- The function of the rhizobial nodABC and nodFEL operons in the biosynthesis of lipo-oligosaccharides.- Chemical signaling of Rhizobium nodulating bean.- Bradyrhizobium japonicum strains produce multiple 2-0-methylfucose-containing lipo-oligosaccharide nodulation signals.- Nod genes and nod factors of Rhizobium species NGR234.- Nod factor production by Azorhizobium caulinodans strain ORS571.- Signals released fromalfalfa regulate microbial activities in the rhizosphere.- Exopolysaccharides and Rhizobium meliloti-alfalfa interactions.- The role of Rhizobium meliloti surface polysaccharides in nodule development.- Rhizobium lequminosarum CE3 lipopolysaccharide structure required in symbiosis with bean.- Pea nod- mutants and nod factor perception.- Biogenesis of peribacteroid membrane (PMB) forming a subcellular compartment essential for symbiotic nitrogen fixation.- Characterization of GmENOD40, a gene expressed in soybean nodule primordia.- Expression of the Medicaqo truncatula ENOD12 gene in response to R. meliloti nod factors and during spontaneous nodulation in transgenic alfalfa.- Molecular interactions during nodule development.- Nodule morphogenesis in the absence of Rhizobium.- Ethylene and nodulation.- Differential gene expression in the development of actinorhizal root nodules.- A cyanobacterial-plant endosymbiosis: Aspects of its establishment.- Potential targets and pathways for Rhizobium’s mitogenic stimulus.- Molecular analysis of components responsible for protection of Azotobacter nitrogenase from oxygen damage.- The regulation of oxygen and its role in regulating nodule metabolism.- In vivo and in vitro oxygen control of nitrogen fixation gene expression in Rhizobium meliloti.- Oxygen sensing by the Rhizobium meliloti two-component regulatory system, FixLJ.- Two oxygen-responsive regulatory cascades control nitrogen fixation genes in Bradyrhizobium japonicum.- Heterocyst differentiation and nitrogen fixation in the cyanobacterium Anabaena.- Regulation of nifA synthesis and activity in Azospirillum brasilense Sp7.- The nifL gene of Azotobactervinelandii: Novel features of sequence, expression and mutant phenotypes.- A maltose-binding protein-nifA (MBP-NIFA)fusion activates transcription from the Klebsiella pneumoniae nifH promoter in a purified system: Stimulation by IHF.- Carbon and nitrogen metabolism in Rhizobium.- Succinate metabolism in Rhizobium meliloti.- Diversion of carbon to polysaccharide synthesis by Bradyrhizobium japonicum and B. elkanii bacteroids.- Molecular control of alfalfa nodule NADH-glutamate synthase: A key for nitrogen assimilation.- Rearrangement of nif operons in cyanobacterial heterocysts.- Gene amplification in Rhizobium.- Molecular phylogeny of rhizobia and their relatives.- The prospects of increasing the input of biological nitrogen fixation in agriculture.- Endophytic diazotrophs in sugar cane, cereals and tuber plants.- Prospects and problems of biological nitrogen fixation in rice production: A critical assessment.- Diversity of photosynthetic Bradyrhizobium strains from stem nodules of Aeschynomene species.- Capacities of Azoarcus sp., a new genus of grass-associated diazotrophs.- Field testing of genetically engineered rhizobia.- Mechanism of nitrogenase catalysis.- Extension of nitrogen fixation to other crops.- Regeneration and transformation of legumes.- Competitiveness of Rhizobium strains for nodule occupancy.- Report from the Taxonomy Meeting, Subcommittee on Aqrobacterium and Rhizobium.- Author Index.