Pain Research: Methods and Protocols

by Z. David Luo (Editor)

Overview

The advancements of medical technology, improvements in medical care, and increased patients’ life span make pain research and related drug development high priorities for both the research community and pharmaceutical companies. Rapid development of basic science research tools, such as techniques of flurometric labeling, genomic and proteomic high throughput screening, and genetically modified animals, promotes the swift acceleration of pain research to a stage allowing integrated investigations of pain processing mechanisms at the single cell and/or molecule level, and in a spatially and temporally controlled manner. Using multidisciplinary approaches, we can dissect the complicity of the sensory circuits connecting peripheral stimulation to maladaptive changes in the sensory pathways as well as pain perceptions at the central nervous system. Pain Research: Methods and Prools, Second Edition provides advanced techniques and animal models that are critical for integrated pain research. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Pain Research: Methods and Prools, Second Edition serves as an ideal guide to novice pain researchers who may not have extensive experiences in the field, or to experienced pain researchers who would like to expand their research in new directions and/or to new mechanisms in different models.

Product Details

ISBN-13:	9781493960958
Publisher:	Springer-Verlag New York, LLC
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #851
Edition description:	Softcover reprint of the original 2nd ed. 2012
Pages:	287
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Advancements in Pain Research.- Genomic Methods for Clinical and Translational Pain Research.- Two-Dimensional Gel Electrophoresis: Discovering Neuropathic Pain-Associated Synaptic Biomarkers in Spinal Cord Dorsal Horn.- Whole-Cell Patch-Clamp Recordings on Spinal Cord Slices.- Whole-Cell Recording in Isolated Primary Sensory Neurons.- Indwelling Supradural Catheters for Induction of Facial Allodynia: Surgical Procedures, Application of Inflammatory Stimuli, and Behavioral Testing.- An Experimental Model of Headache-Related Pain.- A Rodent Model of Trigeminal Neuralgia.- New Models of Experimental Parotitis and Parotid Gland Distension in Rats.- A Rat Pain Model of Facial Cancer.- Orofacial Pain Models and Behavior Assessment.- Unilateral T13 and L1 Dorsal Root Avulsion: Methods for a Novel Model of Central Neuropathic Pain.- A Lumbosacral Ventral Root Avulsion Injury and Repair Model for Studies of Neuropathic Pain in Rats.- A Rat Chronic Pain Model of Spinal Cord Contusion Injury.- The Spared Nerve Injury Model of Neuropathic Pain.- A New Rat Pain Model of Thrombus-Induced Ischemia.- Rat Models of Pancreatitis Pain.- The Monosodium Iodoacetate Model of Osteoarthritis Pain in The Rat.- K/BxN Serum Transfer Arthritis as a Model of Inflammatory Joint Pain.- A New Rat Model of Bone Cancer Pain.- Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury.

Editorial Reviews

From the reviews of the second edition:

“This second edition of Pain Research – Methods and Prools is an excellent compendium of chapters outlining the most current techniques used to study the neurobiology of pain. … Pain Research – Methods and Prools is aimed not only at the novice researcher with little experience in the field but also at bringing new techniques and approaches to the pain research community as a whole. The clear organizational structure of the text makes this a highly achievable goal.” (Bradley Kerr, Canadian Journal of Anesthesia/Journal Canadien d'anesthésie, Vol. 60, January, 2013)

From the Publisher

Reviewer:David B. Glick, MD, MBA(University of Chicago Pritzker School of Medicine)
Description:The book presents a collection of research tools used in pain research. The techniques cover a wide range of experimental models from animal studies to PCR techniques.
Purpose:The purpose is to provide a collection of basic research techniques for the novice pain researcher. The chapters give step-by-step instructions for using a variety of experimental models. These descriptions are slightly more detailed than those normally found in the materials and methods section of a well written journal article. The fairly limited goals set by the authors are reasonably well met in most of the chapters.
Audience:The intended audience is newcomers to pain research, and a person just getting started in pain research might find the broad array of experimental techniques presented a useful introduction to the field of pain research.
Features:Each chapter covers a different technique used by pain researchers. The breadth of techniques covered is an impressive achievement. Unfortunately, some of the chapters are quite short with little more information than what the materials and methods section of a good journal article would cover, and there is significant redundancy across several of the chapters. Additionally, the editing of many of the chapters is poor with frequent spelling errors and incomplete sentences making the text hard to follow.
Assessment:This is probably a useful reference for a person trying to get a sense for the breadth of pain research. Others may find the depth of topic coverage inadequate.