PCR Technology: Principles and Applications for DNA Amplification

by Henry Erlich

Overview

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.

Product Details

ISBN-13:	9780333489482
Publisher:	Palgrave Macmillan UK
Publication date:	10/01/1989
Edition description:	1989
Pages:	246
Product dimensions:	5.98(w) x 9.02(h) x (d)

Table of Contents

Part 1 Introduction to the PCR: history of PCR development; description of the PCR method - basic principles and properties of the PCR; thermostable DNA polymerases; automation; sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR; preparation of specific DNA fragments using PCR; detection of gene expression; genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens; diagnosis of monogenic genetic diseases; HLA DNA typing and disease susceptibility; forensic applications; detection of activated oncogenes; future developments and applications.