Table of Contents
Section I Introduction 1
Chapter 1 Introduction Dennis J. Gray Robert N. Trigiano 3
Section II History of Plant Tissue Culture 7
Chapter 2 History of Plant Tissue and Cell Culture James D. Caponetti Dennis J. Gray Robert N. Trigiano 9
Section III Supporting Methodologies 17
Chapter 3 Getting Started with Tissue Culture: Media Preparation, Sterile Technique, and Laboratory Equipment Caula A. Beyl 19
Chapter 4 Histological Techniques Robert N. Trigiano Kathleen R. Malueg Kimberly A. Pickens Zong-Ming Cheng Effin T. Graham 39
Chapter 5 Photographic Methods for Plant Cell and Tissue Culture Dennis J. Gray 49
Chapter 6 Elements of In Vitro Research Michael E. Compton 55
Chapter 7 A Brief Introduction to Plant Anatomy Robert N. Trigiano Dennis J. Gray 73
Chapter 8 Plant Growth Regulators in Plant Tissue Culture and Development Victor P. Gaba 87
Chapter 9 Software and Databases as Tools for Analyzing Nucleic Acid and Protein Sequences Zhijian T. Li 101
Chapter 10 Molecular Approaches to the Study of Plant Development Albrecht G. von Arnim 119
Section IV Propagation and Development Concepts 143
Chapter 11 Shoot Culture Procedures Michael E. Kane 145
Chapter 12 Propagation from Nonmeristematic Tissues: Organogenesis Otto J. Schwarz Anjuna R. Sharma Robert M. Beaty 159
Chapter 13 Molecular Aspects of In Vitro Shoot Organogenesis Shibo Zhang Peggy G. Lemaux 173
Chapter 14 Propagation from Nonmeristematic Tissues: Nonzygotic Embryogenesis Dennis J. Gray 187
Chapter 15 Some Developmental and Molecular Aspects of Somatic Embryogenesis (Nonzygotic Embryogenesis) Andreas Mordhorst Erika Charbit Sacco C. de Vries 201
Section V Crop Improvement Techniques 211
Chapter 16 Use of Protoplasts for Plant Improvement Richard E. Veilleux Michael E. Compton James A. Saunders 213
Chapter 17 Haploid Cultures Sandra M. Reed 225
Chapter 18 Embryo Rescue Sandra M. Reed 235
Chapter 19 Genetic Engineering Technologies Zhijian T. Li Dennis J. Gray 241
Chapter 20 Genetically Modified Plant Controversies: Sensational Headlines versus Sensible Research Harry A. Richards Laura C. Hudson Matthew D. Halfhill C. Neal Stewart, Jr. 251
Chapter 21 Construction and Use of a Simple Gene Gun for Particle Bombardment Dennis J. Gray Michael E. Compton Ernest Hiebert Chia-Min Lin Victor P. Gaba 265
Chapter 22 A Simple Illumination System for Visualizing Green Fluorescent Protein Dennis J. Gray Subramanian Jayasankar Zhijian T. Li 273
Chapter 23 Germplasm Preservation Leigh E. Towill 277
Chapter 24 Valuable Secondary Products from In Vitro Culture Mary Ann Lila 285
Section VI Special Topics 291
Chapter 25 In Vitro Plant Pathology Subramanian Jayasankar Dennis J. Gray 293
Chapter 26 Variation in Tissue Culture Subramanian Jayasankar 301
Chapter 27 Commercial Laboratory Production Gayle R. L. Suttle 311
Chapter 28 Indexing for Plant Pathogens Alan C. Cassells Barbara M. Doyle 321
Chapter 29 Entrepreneurship for Biotechnology Ventures: From Bench to Bag David W. Altman 333
Index 343
