Protein Engineering and Design

by Paul R. Carey (Editor)

Overview

The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design.

• Introduces and defines the techniques involved in protein engineering and design
• Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides

Product Details

ISBN-13:	9780080539973
Publisher:	Elsevier Science
Publication date:	06/21/1996
Sold by:	Barnes & Noble
Format:	eBook
Pages:	361
File size:	7 MB

About the Author

Carey, Paul R. (Case Western Reserve Univ)

The contributors represent the specialties of biochemistry, chemical physics, chemistry, and pharmacology. Most are from academic medical centers and national institutes in the U.S. Institutions prominently represented include NIH, Univ of Minnesota, Rutgers, Brandeis, and UCSF.

Table of Contents

Prediction:
M.H. Hecht, Strategies for the Design of Novel Proteins.
J. Novotny, Computer Methods in Protein Modeling: An Overview.
Production:
J.R. Rose and C.S. Craik, The Art of Expression: Sites and Strategies for Heterologous Expression.
V.L. MacKay and T. Kelleher, Methods for Expressing Recombinant Proteins in Yeast.
T. Vernet and R. Brousseau, In Vitro Mutagenesis.
Characterization:
G.M. Clore and A.M. Gronenborn, Determination of Structures of Larger Proteins in Solution by Three- and Four-Dimensional Heteronuclear Magnetic Resonance Spectroscopy.
D. Ringe and G.A. Petsko, A Consumer's Guide to Protein Crystallography.
P.R. Carey and W. Surewicz, Spectroscopic and Calorimetric Methods for Characterizing Proteins and Peptides.
Applications:
A. Nathan and J. Kohn, The Design of Polymeric Biomaterials from Natural Alpha-L-Amino Acids.
K.E. McLane, S.J. M.Dunn, A.A. Manfredi, B.M. Conti-Tronconi, and M.A. Raftery, The Nicotinic Acetylcholine Receptor as a Model for a Superfamily of Ligand-Gated Ion Channel Proteins

Editorial Reviews

3 Stars from Doody

Protein engineering and design is a unique and important development of late 20th century science. Dependent on the development of new scientific revolutions are the birth of genetic engineering and the burgeoning discoveries in molecular biology; the invention and development of computer technology, x-ray imaging, and NMR spectroscopy, all of which have facilitated the preparation and analysis of proteins. This book is an excellent up-to-date review of this subject. The prime goal of this book is to introduce and define the techniques that constitute protein engineering. The author has achieved these goals in an excellent fashion. The topic is not only of great interest to the academic community, but to the designers of proteins that have had widespread impact on new products. Examples of these are pharmaceuticals, modified enzymes useful in such industrial uses as detergents, pulp bleaching, and nicotine acetylcholine receptor. The book is unique in its simplicity and straightforward presentation. The references are very up-to-date and pertinent. The reader will obtain a rapid overview of the topics involved and how they contribute to the goals of designing and producing novel proteins with desired properties.

Howard H. Sky-Peck

Outlines the modern methods for designing, producing, and characterizing novel proteins and peptides. Discusses design strategies, computer modeling, heterologous expression, in vitro mutagenesis, protein crystallography, spectroscopic and calorimetric methods for characterization, and other techniques. Also describes polymeric biomaterials and nicotinic acetylcholine receptor as examples of applications. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

Reviewer: Howard H. Sky-Peck, MD, PhD, FRSM (Rush Medical College of Rush University)
Description: Protein engineering and design is a unique and important development of late 20th century science. Dependent on the development of new scientific revolutions are the birth of genetic engineering and the burgeoning discoveries in molecular biology; the invention and development of computer technology, x-ray imaging, and NMR spectroscopy, all of which have facilitated the preparation and analysis of proteins. This book is an excellent up-to-date review of this subject.
Purpose: The prime goal of this book is to introduce and define the techniques that constitute protein engineering. The author has achieved these goals in an excellent fashion.
Audience: The topic is not only of great interest to the academic community, but to the designers of proteins that have had widespread impact on new products. Examples of these are pharmaceuticals, modified enzymes useful in such industrial uses as detergents, pulp bleaching, and nicotine acetylcholine receptor.
Features: The book is unique in its simplicity and straightforward presentation. The references are very up-to-date and pertinent.
Assessment: The reader will obtain a rapid overview of the topics involved and how they contribute to the goals of designing and producing novel proteins with desired properties.

Doody's Review Service